饲粮添加谷氨酰胺对黔东南小香鸡生长性能、免疫性能及肠道黏膜免疫的影响  

Effects of Dietary Glutamine on Growth Performance,Immune Performance and Intestinal Mucosa Immunity of Qiandongnan Xiaoxiang Chickens

作　　者：张柏林[1] 刘宁[1] 刘汝杰 高智席[1] 谢宇潇 王庆容[1] 朱玲 曾伯平[1] ZHANG Bolin;LIU Ning;LIU Rujie;GAO Zhixi;XIE Yuxiao;WANG Qingrong;ZHU Ling;ZENG Boping(Key Laboratory of Conservation and Utilization of Characteristic Animal Resources in Chishui River Basin,Department of Biology and Agriculture of Zunyi Normal College,Zunyi 523006,China)

机构地区：[1]遵义师范学院生物与农业科技学院,赤水河流域特色动物资源保护与利用重点实验室,遵义523006

出　　处：《动物营养学报》2020年第11期5158-5166,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：贵州省科技厅项目(黔科合基础2017[1205]);贵州省高层次创新型人才(遵市科合人才[2017]10号);遵义市校联合基金(遵市科合计[2018]08号);遵义市“15851”人才项目(2050020213#);贵州省教育厅项目(黔教合KY字[2016]258);贵州省服务农村产业革命项目(遵师合农村产业字201905);贵州省普通高等学校产学研合作示范基地(黔教科合KY[343]号)。

摘　　要：本试验旨在研究饲粮添加谷氨酰胺(Gln)对黔东南小香鸡生长性能、免疫性能及肠道黏膜免疫的影响。选择90只1日龄初始体重相近的健康黔东南小香鸡,随机分为3个组,每组5个重复,每个重复6只鸡。对照组饲喂基础饲粮,试验组在基础饲粮的基础上分别添加0.5%和1.0%的Gln。试验期21 d。结果表明:1)与对照组相比,饲粮添加0.5%的Gln显著增加了黔东南小香鸡平均日增重、胸腺指数及血清免疫球蛋白G(IgG)含量(P<0.05)。2)与对照组相比,饲粮添加1.0%的Gln显著增加了黔东南小香鸡平均日采食量及平均日增重(P<0.05),显著降低了料重比(P<0.05),显著增加了胸腺指数、脾脏指数及法氏囊指数(P<0.05),显著增加了血清免疫球蛋白A(IgA)、IgG及免疫球蛋白M(IgM)含量(P<0.05),显著增加了空肠黏膜分泌型免疫球蛋白A(sIgA)含量,显著增加了空肠黏膜黏蛋白1(MUC1)和黏蛋白2(MUC2) mRNA表达水平(P<0.05)。3)1.0%Gln添加组黔东南小香鸡的平均日增重,胸腺指数、脾脏指数、法氏囊指数,血清IgA、IgG及IgM含量,空肠黏膜sIgA含量以及MUC1和MUC2 mRNA表达水平均显著高于0.5%Gln添加组(P<0.05)。由此可见,饲粮添加1.0%的Gln能够提高黔东南小香鸡生长性能、免疫性能及肠道免疫机能。This experiment was to investigate the effects of dietary glutamine(Gln)on growth performance,immune performance and intestinal mucosal immunity of Qiandongnan Xiaoxiang chickens. Ninety healthy 1-day-old Qiandongnan Xiaoxiang chickens with similar initial body weight were randomly allocated into three groups with five replicates per group and six chickens per replicate. Chickens in the control were fed a basal diet,and the others in experimental groups were fed basal diets supplemented with 0.5% and 1.0% Gln,respectively. The experiment lasted for 21 days. The results showed as following:1)compared with the control group,dietary 0.5% Gln significantly increased the average daily gain,thymus index and serum immunoglobulin G(IgG)content of Qiandongnan Xiaoxiang chickens(P<0.05). 2)Compared with the control group,dietary 1.0% Gln significantly increased the average daily gain and average daily feed intake,significantly decreased the feed to gain ratio(P<0.05),significantly increased the thymus index,spleen index and bursa of Fabricius index(P<0.05),significantly increased the contents of immunoglobulin A(IgA),IgG and immunoglobulin M(IgM)in serum(P<0.05),significantly increased the content of secretory immunoglobulin A(sIgA)in jejunal mucosa(P < 0. 05),and significantly increased the mRNA expression levels of mucin 1(MUC1)and mucin 2(MUC2)in jejunal mucosa(P<0.05). 3)The average daily gain,the hymus index,spleen index and bursa of Fabricius index,the contents of IgA,IgG and IgM in serum,and the content of sIgA and mRNA expression levels of MUC1 and MUC2 in jejunal mucosa of 1.0% Gln supplementation group were significantly higher than those of 0.5% Gln supplementation group(P<0.05). In conclusion,dietary 1.0% Gln can improve the growth performance,immune performance and intestinal immune capacity of Qiandongnan Xiaoxiang chickens.

关 键 词：谷氨酰胺 黔东南小香鸡 免疫性能 肠道黏膜免疫 

分 类 号：S831[农业科学—畜牧学]