地肤子提取物对α-葡萄糖苷酶的抑制效应研究  

Inhibitory Effects of Kochia scoparia Extracts on the Activityα-Glucosidase

作　　者：张卫[1] 冯松浩 张雨[1] 王晶晶[1] 李健[1] 王洪涛[1] ZHANG Wei;FENG Songhao;ZHANG Yu;WANG Jingjing;LI Jian;WANG Hongtao(Department of Pharmacy,Zhengzhou Railway Vocational and Technical College,Henan Zhengzhou 451460,China)

机构地区：[1]郑州铁路职业技术学院药学院,河南郑州451460

出　　处：《临床研究》2020年第12期16-18,共3页Clinical Research

基　　金：河南省教育厅高等学校重点科研项目指导计划(18B350010)。

摘　　要：目的研究地肤子提取物石油醚相、乙酸乙酯相、正丁醇相对于α-葡萄糖苷酶所具有的抑制效应及抑酶动力学性质。方法采用PNPG底物模型筛选地肤子提取物以对α-葡萄糖苷酶的活性部位加以抑制,同时通过Lineweave-Burk双倒数方式了解其动力学性质。结果地肤子正丁醇相和乙酸乙酯相具有较高的α-葡萄糖苷酶抑制活性。地肤子正丁醇相的抑制效应显著超过阳性对照组阿卡波糖,正丁醇相和乙酸乙酯相的半数抑制浓度(IC 50)依次是743.54μg/mL、1339.65μg/mL。动力学试验的相关结果揭示,地肤子正丁醇相和乙酸乙酯相的抑酶活性效应类型是非竞争型的。结论地肤子正丁醇相对于α-葡萄糖苷酶具有较强的抑制效应,可以据此加以研究利用。Objective To study the inhibition ofα-glucosidase and its reaction kinetics by the three polar moieties of petroleum ether fraction,ethyl acetate fraction,n-butanol fraction of Kochia scoparia seeds.Methodsα-glucosidase inhibitory fraction in Kochia scoparia extracts was screened by PNPG substrate model,and the dynamic properties were analyzed by Lineweaver-Burk double reciprocal method.Results The Kochia scoparia extracts were all had certain inhibitory effect onα-glycosidase,in which inhibitory effect of n-butanol fraction was obviously higher than that of positive control acarbose,IC 50 were 743.54μg/mL,1339.65μg/mL,respectively.The results of kinetic test showed that the inhibition of n-butanol fraction and ethyl acetate fraction were non competitive.Conclusion The n-butanol extract had significant inhibitory effect onα-glucosidase,which had good value of development and utilization.

关 键 词：地肤子 Α-葡萄糖苷酶 抑制效应 动力学 

分 类 号：R284[医药卫生—中药学]