红汁乳菇液体发酵培养基优化  

The Optimization of Lactarius hatsudake Liquid Fermentation Medium

作　　者：周伟坚[1] 查丽燕 王越 张振伟 文华枢 莫美华[1] ZHOU Wei-jian;ZHA Li-yan;WANG Yue;ZHANG Zhen-wei;WEN Hua-shu;MO Mei-hua(College of Food Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《中国食用菌》2020年第9期22-26,共5页Edible Fungi of China

基　　金：2018年广东省科技创新战略专项资金(重点领域研发计划项目)(2018B020206001);2019年广东省现代农业产业技术体系创新团队建设(2019KJ103)。

摘　　要：在最佳碳源、最佳氮源、最佳无机盐单因素试验的基础上,选取玉米粉、蔗糖、棉籽壳、酵母膏浸汁、麸皮、豆粕粉、石膏、石灰、磷酸二氢钾、磷酸氢二钾作为参试因子,应用均匀设计法,采用U12(1210)均匀设计表进行试验设计,对红汁乳菇的发酵培养基进行了优化。经DPS v9.5数据处理软件,以菌丝体干重为目标函数进行回归分析,得出红汁乳菇液体发酵最佳培养基配方,即1 L培养基中各成分用量为玉米10.91 g、蔗糖3.65 g、棉籽壳41.24g、蛋白胨0.01 g、麸皮26.22 g、豆粕粉4.35 g、石膏1.25 g、石灰0.13 g。Based on the single factor experiment of the best carbon source,the best nitrogen source and the best inorganic salt,corn flour,sucrose,cottonseed hull,yeast extract,wheat bran,soybean meal powder,gypsum,lime,potassium dihydrogen phosphate and potassium hydrogen phosphate were selected as the test factors.The uniform design method was used to design the optimization experiment with U12(1210)uniform design table.By using DPS v9.5 data processing software and taking the dry weight of mycelium as the objective function,the optimal medium for liquid fermentation of Lactarius hatsudake was obtained,that is,corn 10.91 g,sucrose 3.65 g,cottonseed shell 41.24 g,peptone 0.01 g,wheat bran 26.22 g,soybean meal powder 4.35 g,gypsum 1.25 g,lime 0.13 g in 1 liter medium.

关 键 词：红汁乳菇 发酵 培养基 均匀设计 

分 类 号：S646.9[农业科学—蔬菜学]