miR-153下调FOXR2、CDK8和CDK13的表达抑制胶质母细胞瘤细胞增殖  

miR-153 inhibits proliferation of glioblastoma multiforme cell lines by down-regulation of FOXR2,CDK8 and CDK13

作　　者：胡克琦[1] 周达全[1] 陈锋[1] 周毅[1] 敖祥生[1] HU Ke-qi;ZHOU Da-quan;CHEN Feng;ZHOU Yi;AO Xiang-sheng(Department of Neurosurgery,Xiangyang Center Hospital,Xiangyang 441021,China)

机构地区：[1]襄阳市中心医院神经外科,湖北441021

出　　处：《中国临床神经外科杂志》2020年第11期767-770,共4页Chinese Journal of Clinical Neurosurgery

摘　　要：目的探讨miR-153对胶质母细胞瘤细胞增殖的影响。方法体外培养胶质母细胞瘤细胞系U87、U251、SHG-44和T98G细胞,分别转染miR-153质粒(miR-153组)、空载质粒(载体组)和miR-153突变质粒(miR-153突变组),另设置空白对照组(不转染任何质粒)。RT-PCR检测miR-153、FOXR2、CDK8和CDK13的表达,MTT法检测细胞增殖能力。结果miR-153组U87、U251、SHG-44和T98G细胞miR-153表达水平较载体组和空白对照组显著上升(P<0.05),细胞增殖水平较载体组和空白对照组均显著降低(P<0.05)。miR-153组U87、U251、SHG-44和T98G细胞FXOR2、CDK8和CDK13的mRNA水平较miR-153突变组、载体组和空白对照组均显著下降(P<0.05),而后三组之间均无统计学差异(P>0.05)。结论miR-153抑制胶质母细胞瘤细胞增殖,其机制可能与抑制FXOR2、CDK8和CDK13表达有关。Objective To study the effect of miR-153 on the cell proliferation of glioblastoma multiforme(GBM)cell lines and its mechanism.Methods GBM cell lines U87,U251,SHG-44 and T98G were cultured in vitro and transfected with miR-153 overexpression plasmid,vector and miR-153 mutant plasmid,respectively.RT-PCR was used to determine the mRNA level of FOXR2,CDK8 and CDK13.MTT assay was used to determine the proliferation of U87,U251,SHG-44 and T98G cells.Results The level of miR-153 and the mRNA level of FOXR2,CDK8 and CDK13 were significantly up-regulated in U87,U251,SHG-44 and T98G cells(P<0.05),and the proliferation of U87,U251,SHG-44 and T98G cells were inhibited after transfected with miR-153 over-expression(P<0.05).Conclusion miR-153 inhibits proliferation of glioblastoma multiforme cell lines,which may be related to the down-regulation of FOXR2,CDK8 and CDK13.

关 键 词：胶质母细胞瘤 微小核糖核酸 miR-153 细胞增殖 

分 类 号：R739.41[医药卫生—肿瘤] Q786[医药卫生—临床医学]