lncRNA SNHG16对胶质瘤U251细胞增殖、侵袭和迁移的影响  

Effect of lncRNA SNHG16 on proliferation,invasion and migration of glioma U251 cells

作　　者：陈波[1] 云亚滨 杜俊峰[1] 王伟志 CHEN Bo;YUN Ya-bin;DU Jun-feng;WANG Wei-zhi(Department of Neurosurgery,Hohhot First Hospital,Hohhot 010000,China)

机构地区：[1]呼和浩特市第一医院神经外科,内蒙古010000

出　　处：《中国临床神经外科杂志》2020年第11期771-773,共3页Chinese Journal of Clinical Neurosurgery

摘　　要：目的探讨沉默长链非编码RNA核内小RNA宿主基因16(SNHG16)对胶质瘤U251细胞增殖、侵袭和迁移的影响。方法实时荧光定量PCR检测正常胶质细胞HEB、NHA和胶质瘤细胞A172、U251、U87、SHG-4中SNHG16的表达。用siRNA沉默U251细胞SNHG16的表达,分为NC-siRNA组和SNHG16-siRNA组,CCK-8法检测细胞增殖,Transwell实验检测细胞侵袭,划痕实验检测细胞迁移。结果与正常胶质细胞HEB和NHA比较,胶质瘤细胞A172、U251、U87和SHG-4的SNHG16表达水平明显升高(P<0.05)。与NC-siRNA组比较,SNHG16-siRNA组细胞增殖能力、细胞侵袭能力和细胞迁移能力均明显降低(P<0.05)。结论SNHG16在胶质瘤细胞中高表达,特异性沉默SNHG16基因可以抑制胶质瘤细胞的增殖、侵袭和迁移能力。Objective To explore the effect of long-chain noncoding RNA(lncRNA)small nucleolar RNA host gene 16(SNHG16)on the proliferation,invasion and migration of glioma U251 cells.Methods Real-time fluorescence quantitative PCR was used to detect the SNHG16 expression in normal gliocytes HEB and NHA and in glioma cell lines A172,U251,U87 and SHG-4.The NC-siRNA(NCsiRNA group)and SNHG16-siRNA(SNHG16-siRNA group)were transfected into U251 cells.CCK-8 assay was used to detect the cell proliferation.Transwell test was used to detect the cell invasion.Cell scratch test was used to detect the cell migration.Results SNHG16 expression levels of glioma cell lines A172,U251,U87 and SHG-4 were significantly increased compared with normal gliocytes HEB and NHA(P<0.05).Compared with NC-siRNA group,the cell abilities of proliferation,invasion and migration were significantly decreased in SNHG16-siRNA group(P<0.05).Conclusion SNHG16 is highly expressed in glioma cells.SNHG16 gene silencing can inhibit the proliferation,invasion and migration of glioma cells.

关 键 词：胶质瘤 U251细胞 长链非编码RNA 核内小RNA宿主基因16 细胞增殖 细胞侵袭 细胞迁移 

分 类 号：R739.41[医药卫生—肿瘤] Q786[医药卫生—临床医学]