腹主动脉灌注雷帕霉素联合肝动脉化疗治疗肝移植瘤兔模型疗效分析  

Therapeutic effect of intra-arterial infusion of rapamycin combined with hepatic arterial chemotherapy on rabbit models of liver transplantation

作　　者：朱鸷翔[1] 沈松鹤 赵森[1] 王大勇[1] 王志学[2] 李荟苹 ZHU Zhixiang;SHEN Songhe;ZHAO Sen;WANG Dayong;WANG Zhixue;LI Huiping(Radiological Intervention Department First Affiliated Hospital,Henan University,Henan Kaifeng 475001,China;Image Center First Affiliated Hospital,Henan University,Henan Kaifeng 475001,China;Respiratory Medicine,First Affiliated Hospital,Henan University,Henan Kaifeng 475001,China)

机构地区：[1]河南大学第一附属医院放射介入科,河南开封475001 [2]河南大学第一附属医院影像中心,河南开封475001 [3]河南大学第一附属医院,呼吸内科,河南开封475001

出　　处：《现代肿瘤医学》2020年第23期4032-4036,共5页Journal of Modern Oncology

基　　金：河南省医学科技攻关计划(联合共建)项目(编号:LHGJ20190515)。

摘　　要：目的:探究腹主动脉灌注雷帕霉素联合肝动脉化疗治疗肝移植瘤兔模型的疗效。方法:建立肝移植瘤兔模型随机分为对照组和观察组各6只,对照组进行肝动脉栓塞化疗,观察组在此基础上联合动脉灌注雷帕霉素,对比两组模型兔治疗前后的肿瘤体积变化、外周血中CD3+和CD4+细胞的分布以及进行病理学观察即组织切片观察统计肿瘤组织坏死率。结果:治疗之后,两组模型兔均发生了瘤体积的明显减小,并且观察组的瘤体积明显小于对照组;两组模型兔经过治疗后CD3+阳性细胞比例均增加,观察组的CD4+阳性细胞比例相比治疗前增加至(46.8±1.5)%,而对照组无明显变化,并且观察组的CD3+和CD4+阳性比例均明显高于对照组;观察组肿瘤近似圆形,边界清晰,显微镜下观察切片以重度坏死为主[占(50.3±5.7)%],并且明显高于对照组。结论:动脉灌注雷帕霉素联合肝动脉栓塞化疗的疗法对肝移植瘤兔模型的疗效显著,并且增强机体免疫抗癌作用,值得参考。Objective:To investigate the efficacy of intra-arterial infusion of rapamycin combined with hepatic arterial chemotherapy in the treatment of rabbit models of liver transplantation.Methods:Rabbit models of liver transplantation tumors were randomly divided into control group and observation group for 6 models respectively.The control group was treated with hepatic artery embolization chemotherapy.The observation group was combined with arterial infusion of rapamycin.The tumor volume,changes,distribution of CD3^+and CD4^+cells in peripheral blood,and pathological observation,tissue section observation,statistical tumor tissue necrosis rate before and after treatment in rabbits were compared.Results:After treatment,the tumor volume was significantly reduced in both groups of rabbits,and the tumor volume of the observation group was significantly smaller than the control group.The proportion of CD3^+positive cells increased after treatment in both groups.The proportion of CD4^+positive cells in the observation group significantly increased to(46.8±1.5)%,but there was no significant change in the control group,and the proportion of CD3^+and CD4^+positive in the observation group was significantly higher than that in the control group.The tumors in the observation group were approximately circular and the boundary was clear.The sections under the microscope were mainly severely necrotic[occupied(50.3±5.7)%],and significantly higher than the control group.Conclusion:Arterial infusion of rapamycin combined with hepatic artery embolization chemotherapy has a significant effect on the rabbit model of liver transplantation and enhances the body’s immune anticancer effect,which is worthy of reference.

关 键 词：动脉灌注 雷帕霉素 肝动脉栓塞 肝移植瘤兔模型 疗效 

分 类 号：R735.7[医药卫生—肿瘤]