结直肠癌微环境诱导腹膜间皮细胞凋亡的体外实验研究  

Colon cancer microenvironment promotes apoptosis of peritoneal mesothelial cells

作　　者：倪鹏 宋岩[2] 黄宝俊[1] 徐惠绵[1] 王振宁[1] 那迪[1] NI Peng;SONG Yan;HUANG Baojun;XU Huimian;WANG Zhenning;NA Di(Department of Surgical Oncology,First Hospital of China Medical University,Liangning Shenyang 110001,China;Department of ENT,Shengjing Hospital of China Medical University,Liaoning Shenyang 110003,China)

机构地区：[1]中国医科大学附属第一医院肿瘤外科,辽宁沈阳110001 [2]中国医科大学附属盛京医院耳鼻喉科,辽宁沈阳110003

出　　处：《现代肿瘤医学》2020年第23期4061-4065,共5页Journal of Modern Oncology

摘　　要：目的:创建发生腹膜转移早期时结直肠癌对人腹膜间皮细胞HMrSV5的损伤模型,探讨结直肠癌细胞对腹膜间皮细胞的影响及凋亡相关蛋白的表达。方法:将人结直肠癌细胞培养液上清加入到人腹膜间皮细胞系HMrSV5。共培养后,光镜下观察间皮细胞的形态变化、CCK-8检测增殖改变、流式细胞仪判断凋亡比例、Western-blot检测凋亡相关蛋白Bcl-2和Bax、Transwell小室检测受损间皮细胞对结直肠癌细胞的影响。结果:结直肠癌细胞培养液上清接触间皮细胞后,发生了形态改变,细胞的增殖受到了抑制,凋亡蛋白Bcl-2与Bax表达失调。同时,受损间皮能够促进结直肠癌细胞的迁移。结论:结直肠癌细胞在发生腹膜转移的早期时候,就可以通过其分泌物促使间皮细胞发生凋亡,凋亡的发生与Bcl-2和Bax的失调有关;同时受损间皮能够反作用于结直肠癌细胞,促进其迁移。我们从中意识到,保护间皮细胞免受损伤,在预防和治疗结直肠癌腹膜转移中或许能起到一定的作用。Objective:To establish a model of HMrSV5 injury in human peritoneal mesothelial cells in the early stage of peritoneal metastasis,and to investigate the effect of colon cancer cells on peritoneal mesothelial cells and the expression of apoptosis-related proteins.Methods:Human colon cancer cell culture supernatant was added to human peritoneal mesothelial cell line HMrSV5.After co-culture,the morphological changes of mesothelial cells,the proliferation of CCK-8 were detected by light microscopy.The apoptosis rate was determined by flow cytometry.The apoptosis-related proteins Bcl-2 and Bax were detected by Western-blot,and Transwell chamber detected the effects of damaged mesothelial cells on colorectal cells.Results:After exposure to mesothelial cells in colon cancer culture supernatant,morphological changes occurred,cell proliferation was inhibited,and expression of apoptosis proteins Bcl-2 and Bax was dysregulated.At the same time,damaged mesothelium can promote the migration of colorectal cancer cells.Conclusion:In the early stage of peritoneal metastasis,colon cancer cells can promote the apoptosis of mesothelial cells through its secretion.The occurrence of apoptosis is related to the imbalance of Bcl-2 and Bax.At the same time,the damaged mesothelium can counteract colorectal cancer cells and promote their migration.We have realized that protecting mesothelial cells from damage may play a role in the prevention and treatment of peritoneal metastasis of colorectal cancer.

关 键 词：结直肠癌 腹膜转移 间皮细胞 

分 类 号：R735.3[医药卫生—肿瘤]