ZEA与DON单一及联合染毒致仔猪睾丸支持细胞凋亡的影响  

Effect of sertoli cells apoptosis in piglets induced by single or combined administration of zearalenone and deoxynivalenol

作　　者：朱雷 贾北平 曹利 徐静茹 赵杰[1] 冯士彬 李玉[1] 吴金节[1] 王希春[1] ZHU Lei;JIA Beiping;CAO Li;XU Jingru;ZHAO Jie;FENG Shibin;LI Yu;WU Jinjie;WANG Xichun(College of Animal and Technology,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《浙江农业学报》2020年第11期1954-1962,共9页Acta Agriculturae Zhejiangensis

基　　金：国家级大学生创新创业训练计划(201910364045);安徽省生猪产业技术体系资助项目(AHCYJSTX-05-07)。

摘　　要：为研究玉米赤霉烯酮(ZEA)与脱氧雪腐镰刀菌烯醇(DON)单一与联合染毒对仔猪睾丸支持细胞凋亡的作用机理,以10μg·mL^-1 ZEA与0.2μg·mL^-1 DON对睾丸支持细胞单一与联合染毒处理24 h,采用CCK-8法检测细胞活率,电镜观察细胞超微结构变化,流式细胞术、免疫荧光检测细胞凋亡率和线粒体膜电位变化,荧光定量PCR检测凋亡相关基因表达量。结果显示,ZEA与DON单一及联合染毒均可导致仔猪睾丸支持细胞活率下降,细胞超微结构损伤,凋亡率升高,并下调线粒体相关抗凋亡基因Bcl-2表达,上调促凋亡基因Bax、Bid以及下游凋亡相关基因Caspase-3与Caspase-9的表达量,且联合作用大于单一作用。本试验结果表明,ZEA与DON可通过线粒体途径诱导仔猪睾丸支持细胞凋亡,且联合毒性大于单一毒性,表现为亚加性效应。The aim of this study was to investigate the mechanism of apoptosis of sertoli cells in piglets induced by single or combined administration of zearalenone and deoxynivalenol.Sertoli cells were treated with 10μg·mL^-1 ZEA and 0.2μg·mL^-1 DON for 24 h.Cell viability was detected by CCK-8 method,ultrastructural changes were observed by electron microscope,apoptosis rate and mitochondrial membrane potential were detected by flow cytometry and immunofluorescence.The expression of apoptosis related genes was detected by fluorescence quantitative PCR.The results showed that single or combined administration of ZEA and DON could decrease the viability of sertoli cells,damage the ultrastructure of sertoli cells and increase the apoptosis rate.In addition,ZEA and DON could down regulate the mRNA expression of Bcl-2,Bax,Bid,Caspase-3 and Caspase-9.Moreover,the combined effect of ZEA and DON was stronger than single effect.These results showed that ZEA and DON could induce apoptosis of sertoli cells of piglets through mitochondrial pathway,and the combined toxicity was greater than that of single toxicity,showing additive effect.

关 键 词：玉米赤霉烯酮 脱氧雪腐镰刀菌烯醇 睾丸支持细胞 细胞凋亡 

分 类 号：S859.8[农业科学—临床兽医学]