LINC01016在子宫内膜癌组织中的表达及对子宫内膜癌细胞生物学行为的影响  

EXPRESSION OF LINC01016 IN ENDOMETRIAL CARCINOMA AND ITS EFFECT ON THE BIOLOGICAL BEHAVIOR OF ENDOMETRIAL CANCER CELLS

作　　者：李腾 纪新强[2] Li Teng;Ji Xinqiang(Qingdao University,Qingdao 266000,China;不详)

机构地区：[1]青岛大学,山东省青岛市266000 [2]青岛大学医学院附属医院妇产科,山东省青岛市266000

出　　处：《中国煤炭工业医学杂志》2020年第5期462-467,共6页Chinese Journal of Coal Industry Medicine

基　　金：山东省卫生健康委员会基础研究项目(编号:2019JS0054)。

摘　　要：目的探讨LINC01016在子宫内膜癌组织中的表达及对子宫内膜癌细胞恶性生物学行为的影响。方法采用荧光定量PCR(RT-qPCR)及Western blot实验分别检测子宫内膜癌组织与正常子宫内膜组织中LINC01016 mRNA及蛋白表达水平;构建LINC01016过表达及敲低的稳转子宫内膜癌Ishikawa细胞系,RT-qPCR验证转染效率;CCK-8实验检测LINC01016过表达/敲低表达后Ishikawa细胞增殖能力;流式细胞仪检测LINC01016过表达/敲低表达后Ishikawa细胞周期及凋亡能力变化;划痕愈合实验检测LINC01016过表达/敲低表达后Ishikawa细胞迁移能力。结果子宫内膜癌组织中LINC01016 mRNA及蛋白表达较正常组织中明显升高(P<0.05)。细胞实验结果显示,过表达LINC01016后Ishikawa细胞增殖、迁移能力显著增强,G2-M期细胞数显著增多,整体凋亡率显著下降;敲低LINC01016表达后得到相反结果。结论子宫内膜癌组织中LINC01016相对高表达,外源性升高LINC01016表达可促进子宫内膜癌细胞恶性生物学行为发展进程,敲低LINC01016表达则可抑制子宫内膜癌细胞的恶性生物学行为。Objective To investigate the expression of LINC01016 in endometrial cancer and its effect onmalignant biological behavior of endometrial cancer cells.Methods Fluorescence quantitative PCR(RT-qPCR) and Western blot were used to detect the mRNA and protein expression levels of LINC01016 in endometrial carcinoma and normal endometrial tissues respectively.A stable endometrial carcinoma Ishikawa cell line with LINC01016 overexpression and knockdown was constructed and the transfection efficiency was verified by RT-qPCR.CCK-8 assay detected the proliferation ability of Ishikawa cells after LINC01016 overexpression/knockdown expression.Flow cytometry was used to detect the changes of Ishikawa cell cycle and apoptosis ability after LINC01016 overexpression/knockdown expression.The migration ability of Ishikawa cells after LINC01016 overexpression/knockdown expression was detected by scratch healing assay.Results The expression of LINC01016 mRNA and protein in endometrial carcinoma tissues was significantly higher than that in normal tissues(P<0.05).Cell experiment results showed that the proliferation and migration ability of Ishikawa cells were significantly enhanced after LINC01016 overexpression,the number of cells in G2-M stage was significantly increased,and the overall apoptosis rate was significantly decreased;the opposite result was obtained after LINC01016 expression was knocked down.Conclusion The expression of LINC01016 in endometrial cancer tissues was relatively high,and the exogenous elevated expression of LINC01016 could promote the development of malignant biological behaviors of endometrial cancer cells,while the down-regulated expression of LINC01016 could inhibit the malignant biological behaviors of endometrial cancer cells.

关 键 词：LINC01016 子宫内膜癌 生物学行为 

分 类 号：R737.3[医药卫生—肿瘤]