诱导多能干细胞技术及其在疾病研究中的应用  

Induced pluripotent stem cell technology and its application in disease research

作　　者：蔡晨依 孟飞龙 饶琳 刘云玥 赵小立[1] Chenyi Cai;Feilong Meng;Lin Rao;Yunyue Liu;Xiaoli Zhao(Key Research Laboratory for Cell and Gene Engineering of Zhejiang Provincial,Institute of Genetics and Regenerative Biology,College of Life Sciences,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学生命科学学院遗传与再生生物学研究所,浙江省细胞与基因工程重点研究实验室,杭州310058

出　　处：《遗传》2020年第11期1042-1061,共20页Hereditas（Beijing)

基　　金：国家重点基础研究发展规划项目(973计划)(编号:2012CB967900)资助。

摘　　要：自2006年Takahashi和Yamanaka报道生成诱导多能干细胞(induced pluripotent stem cells,iPSCs)以来,多能干细胞领域进入了前所未有的发展状态,在疾病建模、药物发现以及细胞疗法等各方面都发挥重要作用,促进了细胞生物学和再生医学等学科的发展。目前,iPSCs技术已成为研究病理机制的重要工具,利用iPSCs技术筛选的新药物正在研发中,使用iPSCs衍生细胞的临床试验数量也在逐渐增长。iPSCs与基因编辑技术以及3D类器官相结合的最新研究进展促进了iPSCs在疾病研究中的进一步应用。本文介绍了近年来重编程方法的革新,分析了整合病毒载体系统、整合非病毒载体系统、非整合病毒载体系统以及非整合非病毒载体系统四种重编程方法的利弊;同时综述了iPSCs在疾病建模以及临床治疗等方面的最新研究进展,为促进iPSCs各领域的深入研究提供参考。Since Takahashi and Yamanaka reported the generation of induced pluripotent stem cells(iPSCs)in 2006,the field of pluripotent stem cells has entered an unprecedented state of development.It plays an important role in disease modeling,drug discovery and cell therapy,and promotes the development of cell biology and regenerative medicine.At present,iPSC technology has become an important tool for studying of pathological mechanisms.New drugs screened by iPSC technology are being developed,and the number of clinical trials using i PSC-derived cells is gradually increasing.The latest research progress of iPSCs,combined with gene editing technology and 3D organoid methodology,promotes the further applications of iPSCs in disease research.In this review,we introduce the innovation of reprogramming methods in recent years,analyze the advantages and disadvantages of four reprogramming methods:integrated virus vector system,integrated non-viral vector system,non-integrated virus vector system and non-integrated non virus vector system.At the same time,we summarize the latest research progress on iPSCs in disease modeling and clinical treatment strategies,so as to provide a reference for further in-depth research in various fields of iPSCs.

关 键 词：IPSCS 重编程 疾病建模 细胞疗法 

分 类 号：R318[医药卫生—生物医学工程]