“当先实脾”治法对慢性肝损伤模型大鼠脑肠肽的影响  

Effect of“priority spleen strengthening”on brain-gut peptide in rats with chronic liver injury

作　　者：史兴华 郑智礼 于瀚 许宗颖 高艺宁 魏伟莉 邓秀兰 SHI Xinghua;ZHENG Zhili;YU Han;XU Zongying;GAO Yining;WEI Weili;DENG Xiulan(Chinese Medical College of Beijing University of Chinese Medicine,Beijing 100029,China;College of Basic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,Sichuan,China)

机构地区：[1]北京中医药大学中医学院,北京100029 [2]成都中医药大学基础医学院,四川成都610075

出　　处：《现代中西医结合杂志》2020年第33期3649-3653,3707,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(81573963);北京中医药大学自主课题(2019-JYB-XS-009)。

摘　　要：目的探讨“当先实脾”治法对肝郁叠加慢性肝损伤大鼠模型脑肠肽的影响。方法采用随机数字表法将36只清洁级雄性SD大鼠分为正常组、模型组、双环醇组、疏肝健脾组、当先治肝组、当先实脾组,每组6只。除正常组外,其余组均采用慢性束缚和皮下注射四氯化碳的方法建立肝郁叠加慢性肝损伤模型。造模同时,双环醇组给予双环醇灌胃42 d,疏肝健脾组给予柴芍六君子汤灌胃42 d,当先治肝组给予四逆散灌胃21 d+柴芍六君子汤灌胃21 d,当先实脾组给予六君子汤灌胃21 d+柴芍六君子汤灌胃21d,正常组和模型组给予等容量蒸馏水灌胃42 d。观察各组大鼠实验过程中一般状态,实验结束后测定各组大鼠血清中丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、总胆红素(TBil)、二胺氧化酶(DAO)、胃泌素(GAS)、P物质(SP)水平。取肝组织,HE染色观察肝组织病理变化,RT-PCR法检测大鼠结肠和下丘脑中血管活性肠肽(VIP)、胃饥饿素(Ghrelin)mRNA表达情况。结果与正常组比较,模型组大鼠血清ALT、AST、TBil、DAO水平和结肠、下丘脑中VIP mRNA表达量均明显升高(P均<0.05),血清GAS、SP水平和结肠、下丘脑中Ghrelin mRNA表达量均明显降低(P均<0.05)。与模型组比较,双环醇组、当先实脾组大鼠血清ALT、AST、TBil、DAO水平和结肠、下丘脑中VIP mRNA表达量均明显降低(P均<0.05),血清GAS、SP水平和结肠、下丘脑中Ghrelin mRNA表达量均明显升高(P均<0.05)。结论“当先实脾”治法可明显改善肝郁叠加慢性肝损伤,机制可能与调节脑肠肽表达有关。Objective It is to explore the effect of therapy for“Priority spleen strengthening”on brain intestinal peptide in rats with liver depression and chronic liver injury.Methods 36 clean-grade male SD rats were randomly divided into 6 groups,including normal group,model group,bicyclol group,soothing liver and strengthening spleen group,priority liver soothing group and priority spleen strengthening group,with 6 rats in each group.Except for the normal group,the other groups were given chronic binding and subcutaneous injection of carbon tetrachloride(CCL4)to establish the models of liver depression and chronic liver injury.At the same time of modeling,the bicyclol group was treated with bicyclol by gavage for 42 days,the soothing liver and strengthening spleen group was treated with Chaishao Liujunzi Decoction by gavage for 42 days,and the priority liver soothing group was treated with Sinisan Decoction for 21 d+Chaishao Liujunzi Decoction for 21 days by gavage,and the priority spleen strengthening group was treated with Liujunzi Decoction for 21 days+Chaishao Liujunzi Decoction for 21 days,the normal group and the model group were treated with an equal volume of distilled water for 42 days.The general state of rats in each group during the experiment were observed.After the experiment,the levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBil),and diamine oxidase(DAO),gastrin(GAS),substance P(SP)in the rats of each group were determined.The liver tissues were taken to observe the pathological changes of liver tissues by HE staining,the expression of vasoactive intestinal peptide(VIP)and ghrelin(Ghrelin)mRNA in the colon and hypothalamus of rats were detected by RT-PCR.Results Compared with the normal group,the levels of serum ALT,AST,TBil,DAO and the VIP mRNA expression in colon and hypothalamus of the model group were significantly increased(P<0.05),the levels of serum GAS and SP and the expression Ghrelin mRNA in the colon and hypothalamus were significantly decrease

关 键 词：慢性肝损伤 当先实脾 肝郁 柴芍六君子汤 脑肠肽 

分 类 号：R-332[医药卫生]