茎瘤芥BXL基因家族的全基因组鉴定及在瘤状茎膨大过程中的表达分析  

Genome-wide identification of BXL family genes in Brassica juncea var.tumida and their expression during swelling of stem

作　　者：赖彪 陈春帆 刘春渝 朱纯真 杜丽娜 LAI Biao;CHEN Chunfan;LIU Chunyu;ZHU Chunzhen;DU Li’na(School of Advanced Agriculture and Bioengineering,Yangtze Normal University,Chongqing 408100,China)

机构地区：[1]长江师范学院现代农业与生物工程学院,重庆408100

出　　处：《华中农业大学学报》2020年第6期155-163,共9页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31701928);重庆市科技局基础科学与前沿技术研究专项(cstc2017jcyjAX0226);重庆市教委科学技术研究项目(KJ1712305);长江师范学院校级科研项目(2016KYQD19和2016XJQN07)。

摘　　要：为深入解析BXL家族基因在茎瘤芥发育中的功能,利用生物信息学方法从茎瘤芥全基因组鉴定出16个BjuBXL,并系统分析了BjuBXL家族蛋白的理化性质、基因结构、保守基序、染色体定位和在茎膨大过程中的表达。结果表明:16个BjuBXL基因分为7类,共2个亚族,其中BjuBXL1-1~BjuBXL1-4与拟南芥中参与细胞壁代谢和植物发育的AtBXL1亲缘关系相近;7个BjuBXL定位于A亚基因组的5条染色体上,8个定位于B亚基因组的4条染色体上;荧光定量PCR分析表明BjuBXL1-1和BjuBXL1-2在茎膨大过程中均明显上调,而且在茎的内髓区(P2~P4)表达量均显著高于外髓区(P1),可能参与茎膨大过程中茎内髓区的细胞壁代谢。In order to further analyze the functions of BXL family genes in the development of stem,16 genes encoding putative BXL proteins were identified in the whole genome of Brassica juncea var.tumida with bioinformatics methods.The gene structure and conserved motifs,chromosomal distribution and gene expression during the swelling of stem of BjuBXL family genes and the physicochemical properties of BjuBXL family protein were systematically analyzed.The results showed that 16 BjuBXL genes were classified into 7 categories and 2 sub-families according to the phylogenetic tree analysis,among which BjuBXL1-1,BjuBXL1-2,BjuBXL1-3 and BjuBXL1-4 are closely related to AtBXL1,which is involved in cell wall metabolism and plant development in Arabidopsis.7 BjuBXL genes were located in 5 chromosomes of A subgenomes and 8 BjuBXL genes are located in 4 chromosomes of B subgenomes.The results of fluorescence quantitative PCR analyses showed that the transcriptional levels of BjuBXL1-1and BjuBXL1-2 were up-regulated obviously during the swelling of stem.The expression in the inner pith(P2-P4)were significantly higher than that in regions of the outer pith of swollen stem(P1),indicating that BjuBXL1-1 and BjuBXL1-2 may play an essential role in the cell wall metabolism of inner pith during the swelling of stem.

关 键 词：茎用芥菜 茎瘤芥(Brassica juncea var.tumida) BXL 转录组 茎膨大 品质改良 

分 类 号：S637.3[农业科学—蔬菜学]