Evaluation of circulating tumor DNA as a biomarker for gynecological tumors  

作　　者：Kang-Sheng Liu Hua Tong Tai-Ping Li Ya-Jun Chen 

机构地区：[1]Department of Clinical Laboratory,Women's Hospital of Nanjing Medical University,Nanjing Maternity and Child Health Care Hospital,Nanjing,Jiangsu 210029,China [2]Department of Obstetrics and Gynecology,Women's Hospital of Nanjing Medical University,Nanjing Maternity and Child Health Care Hospital,Nanjing,Jiangsu 210029,China [3]Department of Neuro-Psychiatric Institute,The affiliated Brain Hospital,Nanjing Medical University,Nanjing,Jiangsu 210029,China

出　　处：《Chinese Medical Journal》2020年第21期2613-2615,共3页中华医学杂志（英文版）

摘　　要：Circulating tumor DNA(ctDNA)is a DNA fragment released into the peripheral blood by apoptotic or necrotic tumor cells,which carry the basic genetic information after gene mutation.The detection and analysis of ctDNA can provide genomic information on the size and development of tumor;therefore,it is considered as an emerging and promising tumor biomarker for cancer progression,reoccurrence,and routine monitoring after surgery.Albeit the isolation method is relatively simple due to the stability of ctDNA,the abundance of ctDNA is extremely low,and result from the high content of background cell free DNA(cfDNA),the large difference between individuals,and the need of predicted detection sites,it is necessary to analyze ctDNA in a comprehensive way.At present,the main methods that meet the needs of ctDNA detection with sensitivity and specificity are amplification refractory mutation system PCR(ARMS-PCR),digital-PCR(dPCR)and high-throughput sequencing.Among them,dPCR has advantages in practicality,which can realize absolute quantification of single-molecule DNA,detect and trace DNA molecules and conduct quantitative analysis,providing a reliable basis for clinical monitoring of tumor recurrence and minimal residual diseases.The study reviews the detection and potential value of ctDNA in gynecologic tumors.

关 键 词：ROUTINE SPECIFICITY ABSOLUTE 

分 类 号：R737.3[医药卫生—肿瘤]