KCTD10抑制非小细胞肺癌中Hippo通路活性  

KCTD10 inhibits Hippo pathway activity in non-small cell lung cancer

作　　者：杨曼 韩雅梅 刘晓芳[1,2] 林旭勇 YANG Man;HAN Yamei;LIU Xiaofang;LIN Xuyong(Department of Pathology,the First Affiliated Hospital of China Medical University,Liaoning Shenyang 110001,China;Department of Pathology,College of Basic Medical Sciences,China Medical University,Liaoning Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院病理科,辽宁沈阳110001 [2]中国医科大学基础医学院病理学教研室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2020年第24期4215-4219,共5页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:81401885);辽宁省自然科学基金(编号:20180530040)。

摘　　要：目的:检测KCTD10在非小细胞肺癌中的表达水平及其对Hippo通路的影响。方法:采用免疫组化方法检测KCTD10在非小细胞肺癌组织标本中的表达;采用Western blot检测YAP、P-YAP、P-LATS1、MMP7、CyclinE在非小细胞肺癌细胞中的表达;采用集落形成实验和Transwell实验检测细胞增殖和迁移。结果:KCTD10在非小细胞肺癌组织及细胞系中高表达;KCTD10过表达促进A549细胞增殖和迁移;过表达KCTD10的A549细胞系YAP的表达总量未见明显变化,但抑制了P-YAP、P-LATS1的表达,促进了MMP7、CyclinE的表达。结论:KCTD10抑制了非小细胞肺癌中Hippo通路活性;KCTD10可能作为非小细胞肺癌防治的一个潜在靶向分子。Objective:To detect the expression level of KCTD10 in non-small cell lung cancer and its influence on the Hippo pathway.Methods:Immunohistochemical method was used to detect the expression of KCTD10 in non-small cell lung cancer tissue samples.Western blot was used to detect the expression of YAP,P-YAP,P-LATS1,MMP7,and CyclinE in non-small cell lung cancer cells.Colony formation and the Transwell assay were used to detect the proliferation and migration ability of cells.Results:KCTD10 was highly expressed in non-small cell lung cancer tissues and cell lines.KCTD10 overexpression promoted the proliferation and migration of A549 cells.The total expression of YAP in KCTD10-overexpressing A549 cell line did not change significantly,but it suppressed the expression of P-YAP and P-LATS1,and promoted the expression of MMP7 and CyclinE.Conclusion:KCTD10 inhibits the activity of Hippo pathway in non-small cell lung cancer.KCTD10 may be a potential targeting molecule for the prevention and treatment of non-small cell lung cancer.

关 键 词：KCTD10 非小细胞肺癌 Hippo通路 

分 类 号：R734.2[医药卫生—肿瘤]