小儿视网膜母细胞瘤VEGF、Cyclin D1及E-Cadherin表达及与组织病理特征的关系  

Expression of VEGF,Cyclin D1 and E-Cadherin in pediatric retinoblastoma and their relationship with histopathological characteristics

作　　者：汪岭 马雪莲 王启明 WANG Ling;MA Xuelian;WANG Qiming(Department of Ophthalmology,Maternal and Child Health Hospital of Qiaokou District,Wuhan,Hubei,China,430030;Department of Laboratory Medicine,Dongying District People's Hospital,Dongying City,Dongying,Shandong,China,257000;Department of Ophthalmology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei,China,430022)

机构地区：[1]武汉市硚口区妇幼保健院眼科,湖北武汉430030 [2]东营市东营区人民医院检验科,山东东营257000 [3]华中科技大学同济医学院附属协和医院眼科,湖北武汉430022

出　　处：《分子诊断与治疗杂志》2020年第11期1452-1456,共5页Journal of Molecular Diagnostics and Therapy

基　　金：东营市卫生计生委科技项目(2015BWDY004)。

摘　　要：目的探究小儿视网膜母细胞瘤(RB)组织中的血管内皮生长因子(VEGF)、细胞周期调节蛋白(Cyclin D1)及上皮钙黏蛋白(E-Cadherin)表达及与组织病理特征的关系。方法选取本院2017年1月至2019年1月收集的55例小儿RB组织样本及正常视网膜组织样本,免疫组化SP法检测并对比VEGF、Cyclin D1及E-Cadherin表达情况,分析VEGF、Cyclin D1及E-Cadherin表达相关性及三指标与患儿组织病理特征的关系。结果免疫组化染色结果显示,RB组织中VEGF、Cyclin D1表达阳性率均显著高于正常视网膜组织,E-Cadherin表达显著低于正常视网膜组织,差异有统计学意义(P<0.05)。Spearman相关性分析结果显示,VEGF、Cyclin D1及E-Cadherin表达存在正相关性(P<0.05)。RB患儿不同性别、年龄、眼别VEGF、Cyclin D1及E-Cadherin表达阳性率比较,差异无统计学意义(P>0.05);不同分化情况、临床分期、视神经侵犯程度、病理分期RB患儿VEGF、Cyclin D1及E-Cadherin表达阳性率比较,差异有统计学意义(P<0.05)。Cox多因素分析结果显示,临床分期越高、神经侵犯程度越高、VEGF、Cyclin D1高表达及E-Cadherin低表达为影响患者生存的不良因素(P<0.05)。结论 VEGF、Cyclin D1阳性表达越高、E-Cadherin阳性表达越低,小儿RB肿瘤恶性程度越高、预后越差,检测VEGF、Cyclin D1及E-Cadherin表达有助于RB患儿病情评估。Objective To investigate the expression of vascular endothelial growth factor(VEGF),cell cycle regulatory protein(Cyclin D1)and epithelial cadherin(E-Cadherin)in pediatric retinoblastoma(RB)tissues and their relationship with histopathological characteristics.Methods 55 tissue samples from pediatric RB patientsand normal controls were collected in our Hospital from January 2017 to January 2019.Immunohistochemical SP method was used to detect and compare the expression of VEGF,Cyclin D1 and E-Cadherin,and analyze VEGF,Cyclin D1 and E-Cadherin expression correlation and the relationship between the three indicators and histopathological characteristics of children.Results The results of immunohistochemical staining showed that the positive rates of VEGF and Cyclin D1 expression in the RB tissues were significantly higher than those in the normal retinal tissues,and the expression of E-Cadherin was significantly lower than that in the normal retinal tissues.The difference was statistically significant(P<0.05).Spearman correlation analysis showed that the expression of VEGF,Cyclin D1 and E-Cadherin were positively correlated(P<0.05).There was no statistically significant difference in the positive rates of VEGF,Cyclin D1 and E-Cadherin expression in children with RB in different genders,ages,eye categories(P>0.05).However,there was significant difference in the positive rates of VEGF,Cyclin D1 and E-Cadherin expression in children with different differentiation,clinical stage,optic nerve invasion and pathological stage were compared with statistical significance(P<0.05).The results of Cox multivariate analysis showed that the higher the clinical stage,the higher the degree of neurological invasion,and the high expression of VEGF,Cyclin D1 and the low expression of E-Cadherin were the adverse factors affecting the survival of patients(P<0.05).Conclusion The higher the positive expression of VEGF and Cyclin D1 and the lower the positive expression of E-Cadherin,the higher the malignant degree of RB tumors in children,a

关 键 词：小儿 视网膜母细胞瘤 血管内皮生长因子 细胞周期调节蛋白 上皮钙黏蛋白 

分 类 号：R739.7[医药卫生—肿瘤]