甲状腺癌细针穿刺组织中CEACAM1表达量及其与肿瘤恶性程度的相关性  

Expression level of CEACAM1 in fine needle aspiration tissue of thyroid cancer and its correlation with tumor malignancy

作　　者：徐显昌[1] 周宁[2] 陈志刚 XU Xianchang;ZHOU Ning;CHEN Zhigang(Department of General Surgery,Jiangyou Hospital of Traditional Chinese Medicine,Mianyang,Sichuan,China,621700;Department of Pathology,404 Hospital,Mianyang,Sichuan,China,621000)

机构地区：[1]四川省绵阳市江油市中医医院普外科,四川绵阳621700 [2]四川绵阳四0四医院病理科,四川绵阳621000

出　　处：《分子诊断与治疗杂志》2020年第11期1544-1547,1551,共5页Journal of Molecular Diagnostics and Therapy

基　　金：四川省中医药管理局重点项目(2019F015)。

摘　　要：目的分析甲状腺癌细针穿刺组织中癌胚抗原黏附分子1(CEACAM1)表达量及其与肿瘤恶性程度的相关性。方法选取2018年04月至2020年4月于本院行甲状腺结节细针穿刺活检的118例患者为研究对象,根据穿刺后病理检查结果分为甲状腺癌55例(甲状腺癌组)和良性甲状腺肿瘤63例(甲状腺良性肿瘤组),测定CEACAM1表达量、血清肿瘤标记物[细胞角蛋白19片段(Cyfra21-1)、半乳糖血凝素-3(Cal-3)、癌胚抗原(CEA)、基质金属蛋白酶-9(MMP-9)]、增殖基因[叉头框蛋白A1(FOXA1)、瘦素受体(ObR)、增殖细胞核抗原(PCNA)]和侵袭基因[BCL6共抑制因子样蛋白1(BCORL1)、S100钙结合蛋白A4(S100A4)、特异AT序列结合蛋白1(SATB1)、人Twist相关蛋白1(Twist1)]表达量,并分析CEACAM1与Cyfra21-1、Cal-3、CEA、MMP-9、FOXA1、ObR、PCNA、BCORL1、S100A4、SATB1、Twist1的相关性。结果甲状腺癌组CEACAM1表达量和阳性率高于甲状腺良性肿瘤组,差异具有统计学意义(P<0.05);甲状腺癌组血清Cyfra21-1、Cal-3、CEA及MMP-9水平和FOXA1、ObR、PCNA、BCORL1、S100A4、SATB1、Twist1蛋白表达量高于甲状腺良性肿组,差异具有统计学意义(P<0.05);Spearman相关分析显示CEACAM1表达量与Cyfra21-1、Cal-3、CEA、MMP-9、FOXA1、ObR、PCNA、BCORL1、S100A4、SATB1、Twist1呈正相关性(P<0.05)。结论甲状腺癌细针穿刺组织中CEACAM1呈高表达,且与肿瘤恶性程度呈正相关。Objective To analyze the expression level of carcinoembryonic antigen related cell adhesion molecule 1(CEACAM1)in fine needle aspiration tissue of thyroid cancer and its correlation with tumor malignancy.Methods A total of 118 patients who underwent fine needle aspiration biopsy of thyroid nodules in the hospital from April 2018 to April 2020 were enrolled as the research objects.According to the pathologic examination results after puncture,there were 55 cases of thyroid cancer(thyroid cancer group)and63 cases of benign thyroid tumor(thyroid benign tumor group).The expression levels of CEACAM1,serum tumor markers[cytokeratin 19 fragment(Cyfra21-1),galactose hemagglutinin-3(Cal-3),carcinoembryonic antigen(CEA),matrix metalloproteinase-9(MMP-9)],proliferation genes[forkhead box protein A1(FOXA1),leptin receptor(ObR),proliferating cell nuclear antigen(PCNA)]and invasion genes[BCL6 corepressor protein 1(BCORL1),S100 calcium binding protein A4(S100 A4),specific AT sequence binding protein 1(SATB1),human Twist related protein 1(Twist1)]were detected.The correlation between CEACAM1 and Cyfra21-1,Cal-3,CEA,MMP-9,FOXA1,ObR,PCNA,BCORL1,S100 A4,SATB1,Twist1 was analyzed.Results The expression level and positive rate of CEACAM1 in the thyroid cancer group were higher than those in the benign thyroid tumor group.The difference was statistically significant(P<0.05).The levels of serum Cyfra21-1,Cal-3,CEA and MMP-9,FOXA1,ObR,PCNA,BCORL1,S100 A4,SATB1 and Twist1 in the thyroid cancer group were higher than those in benign thyroid tumor group.The difference was statistically significant(P<0.05).Spearman correlation analysis showed that expression level of CEACAM1 was positively correlated with Cyfra21-1,Cal-3,CEA,MMP-9,FOXA1,ObR,PCNA,BCORL1 S100 A4,SATB1 and Twist1(P<0.05).Conclusion CEACAM1 is highly expressed in fine needle aspiration tissue of thyroid cancer,and is positively correlated with the degree of tumor malignancy.

关 键 词：甲状腺癌 细针穿刺组织 癌胚抗原黏附分子1 肿瘤恶性程度 

分 类 号：R736.1[医药卫生—肿瘤]