HE和Giemsa染色在儿童细菌性和过敏性结膜炎诊断中的比较  

Comparison of HE and Giemsa staining in diagnosis of bacterial and allergic conjunctivitis in children

作　　者：高娜 侯磊 郝雪莲 亢泽峰[3] Na Gao;Lei Hou;Xue-Lian Hao;Ze-Feng Kang(Department of Ophthalmology, Second People's Hospital of Xining City, Xining 810000, Qinghai Province, China;Department of Ophthalmology, First People's Hospital of Xining City, Xining 810000, Qinghai Province, China;Eye Hospital of China Academy of Chinese Medical Sciences, Beijing 100040, China)

机构地区：[1]中国青海省西宁市第二人民医院眼科,810000 [2]中国青海省西宁市第一人民医院眼科,810000 [3]中国中医科学院眼科医院,中国北京市100040

出　　处：《国际眼科杂志》2020年第12期2178-2180,共3页International Eye Science

摘　　要：目的:探讨苏木精-伊红染色法(HE)和吉姆萨(Giemsa)染色法在儿童细菌性和过敏性结膜炎中的诊断效果。方法:选取2016-10/2019-10期间于我院眼科经结膜刮片检查诊断为结膜炎儿童患者422例作为研究对象,分别采用HE与Giemsa染色法进行结膜刮片染色,并根据染色结果诊断细菌性/过敏性结膜炎。观察两种染色结果对细菌性/过敏性结膜炎的阳性检出率及染色情况。结果:HE染色诊断细菌性结膜炎阳性率(33.0%)和符合率(63.6%)显著低于Giemsa染色(90.7%和88.8%,均P<0.001),而过敏性结膜炎阳性率无明显差异(90.8%vs 87.2%,P>0.05)。结论:Giemsa染色法检查可准确诊断儿童细菌性结膜炎且方法简便,HE与Giemsa染色法均对过敏性结膜炎具有较好的诊断效果,可为提高临床诊断效率及早期治疗方案提供依据。AIM:To explore the diagnostic effect of hematoxylin-eosin staining(HE)and Giemsa staining in the diagnosis of bacterial and allergic conjunctivitis in children.METHODS:Totally 422 children with conjunctivitis diagnosed by conjunctivitis from the ophthalmology department of our hospital during 2016-10/2019-10 as the research objects.HE and Giemsa staining methods were used to stain the conjunctival scratches,and the staining results were used to diagnose bacterial/allergic conjunctivitis.Observe the positive detection rate of the two staining results for bacterial/allergic conjunctivitis and the staining situation.RESULTS:The positive rate(33.0%)and coincidence rate(63.6%)of HE staining for the diagnosis of bacterial conjunctivitis were significantly lower than Giemsa staining(90.7%and 88.8%,P<0.001),while the positive rate of allergic conjunctivitis was not significantly different (90.8%vs 87.2%,P>0.05).CONCLUSION:The Giemsa staining method can accurately diagnose bacterial conjunctivitis in children and the method is simple.Both HE and Giemsa staining methods have good diagnostic effects on allergic conjunctivitis,which can provide a basis for improving the clinical diagnosis efficiency and early treatment options.

关 键 词：苏木精-伊红染色 吉姆萨染色 细菌性结膜炎 过敏性结膜炎 鉴别诊断 

分 类 号：R777.31[医药卫生—眼科]