二倍体马铃薯绿原酸的提取与光谱测定分析  

Extraction and Spectroscopic Analysis of Chlorogenic Acid in Diploid Potato

作　　者：杨慧芹 张博[1,2] 马玲 尚轶 高冬丽 YANG Hui-qin;ZHANG Bo;MA Ling;SHANG Yi;GAO Dong-li(Yunnan Key Laboratory of Potato Biology,Kunming 650500,China;Joint Academy of Potato Science,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南省马铃薯生物学重点实验室,云南昆明650500 [2]云南师范大学马铃薯科学研究院,云南昆明650500

出　　处：《光谱学与光谱分析》2020年第12期3860-3864,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金面上项目(31972433);云南师范大学博士研究生创新基金项目(yjs2018151)资助。

摘　　要：马铃薯营养成分全面、营养结构合理、易于消化吸收,是我国第四大粮食作物。马铃薯中富含绿原酸等酚类物质,具有抗氧化、抗病、抗癌、降脂等多种保健作用。鉴于绿原酸与马铃薯风味、营养及抗性品质密不可分,系统测定分析马铃薯不同组织中的绿原酸含量及分布规律将为改良作物品质提供理论依据和借鉴。马铃薯普通栽培种是同源四倍体,应用杂交育种的方法改良品种比较困难,而二倍体马铃薯比四倍体马铃薯遗传背景简单,在育种和基因组研究方面更具优势。采用二倍体马铃薯自然变异群体(C系列)和自交分离F2群体(151系列和RH系列)为实验材料,利用紫外-可见光分光光度计(UV-VIS)对不同组织中绿原酸含量进行测定计算,并采用SPSS16.0对绿原酸测定结果进行统计分析。结果表明,在所选取的131个样本中, C系列马铃薯叶片绿原酸含量明显高于151系列和RH系列, C系列马铃薯叶片绿原酸含量最高为12 022.25 mg·kg^-1 FW, 151系列马铃薯叶片绿原酸含量最高为7 685.41 mg·kg^-1 FW, RH系列马铃薯叶片绿原酸含量最高为5 486.46 mg·kg^-1 FW,但C系列均匀性和稳定性低于151系列和RH系列。进一步对C系列(181个样本)薯皮和薯肉绿原酸含量进行测定分析,薯皮中绿原酸含量明显高于薯肉,薯皮中绿原酸含量最高为19 679.59 mg·kg^-1 DW,薯肉中绿原酸含量最高为2 927.52 mg·kg^-1 DW,整体上薯肉中绿原酸含量随薯皮绿原酸含量的增加而增加,但不呈线性关系。通过对二倍体马铃薯绿原酸含量的测定分析,为光谱测定分析法在研究、培育绿原酸含量高、风味好的马铃薯新品种中的应用提供理论基础。同时,光谱测定分析结果对指导育种、天然活性物质的测定分析及农业基因组研究具有重要的意义,也为紫外-可见光谱在国家优薯计划、国际农业基因组计划和智慧农业领域的应用提供研究和技术�Potato is the fourth largest food crop in China,which has a comprehensive and reasonable nutrient composition and is easy to digest and absorb.Potato tuber is rich in phenolic substances such as chlorogenic acid,which has many health functions such as anti-oxidation,disease resistance,anti-cancer and lipid-lowering.Since chlorogenic acid is closely related to potato flavor,nutrition and resistance quality,systematic determination and analysis of chlorogenic acid content and distribution in different tissues of potato will provide a theoretical basis and reference for improving crop quality.The common cultivars of potato are autotetraploid,so it is difficult to improve potato varieties by cross breeding.While the diploid potato has more advantages in breeding and genome research due to its simple genetic background compared with the tetraploid potato.In this paper,natural variation population(C series)and self-cross separation of F2 population(151 series and RH series)of diploid potatoes were used.The content of chlorogenic acid in different tissues was determined by UV-Vis and the results were statistically analyzed by SPSS16.0.The results showed that,among the 131 samples selected,the chlorogenic acid content in potato leaves of series C is significantly higher than 151 series and RH series.The highest chlorogenic acid content in potato leaves of series C is 12022.25 mg·kg-1 FW,the highest chlorogenic acid content in potato leaves of series 151 is 7685.41 mg·kg-1 FW and the highest chlorogenic acid content in potato leaves of series RH is 5486.46 mg·kg-1 FW.However,the uniformity and stability of C series are lower than 151 series and RH series.The content of chlorogenic acid in potato skin and flesh of C series(181 samples)was further determined and analyzed.The content of chlorogenic acid in potato skin is significantly higher than flesh.The highest content of chlorogenic acid in potato skin is 19679.59 mg·kg-1 DW,while the highest content of chlorogenic acid in potato flesh is 2927.52 mg·kg-1 DW.On the w

关 键 词：马铃薯 绿原酸 紫外光谱 测定 统计分析 

分 类 号：S532.0[农业科学—作物学]