摩腹力学刺激对腹泻型肠易激综合征大鼠肠神经系统的影响  

Effect of abdominal mechanical stimulation on nervous system in irritable bowel syndrome-Diarrehea

作　　者：骆雄飞[1] 刘斯文[1] 李华南[1] 张玮[1] 王金贵[1] 黎波[1] 王强松[2] Luo Xiongfei;Liu Siwen;Li Huanan;Zhang Wei;Wang Jingui;Li Bo;Wang Qiangsong(Department of Tuina,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Laboratory for Biological Effects of Tuina Manipulation of State Administration of Traditional Chinese Medicine of the People's Republic of China,Tianjin 300193,China;Institute of Biomedical Engineering,Chinese Academy of Medical Science&Peking Union Medical College,Tianjin 300192,China)

机构地区：[1]天津中医药大学第一附属医院推拿科,国家中医药管理局推拿手法生物效应三级实验室,300193 [2]中国医学科学院生物医学工程研究所,天津300192

出　　处：《国际生物医学工程杂志》2020年第2期94-99,共6页International Journal of Biomedical Engineering

基　　金：国家自然科学基金面上项目(81674093,81873394);中央高校基本科研业务专项(3332020059)。

摘　　要：目的观察摩腹力学刺激对腹泻型肠易激综合征(IBS-D)大鼠的干预治疗作用,探讨摩腹力学刺激改善IBS-D的可能机制。方法将60只新生Wistar大鼠按随机数字表法分为空白组、模型组和摩腹组,每组20只。模型组和摩腹组均采用新生期母子分离方法建立IBS-D大鼠模型。摩腹组大鼠建模后给予摩腹力学刺激干预,每天干预1次,连续14 d;模型组大鼠建模后每天被仰卧束缚于实验台上5 min,不予摩腹力学刺激干预,连续14 d;空白组大鼠不造模,不予任何干预。通过测定玻璃小球排出时间和大鼠腹部抬起容量阈值分别评估大鼠的肠动力和内脏敏感性,同时采用苏木精-伊红染色观察大鼠结肠组织的病理变化,甲苯胺蓝染色观察大鼠结肠组织中肥大细胞的形态和数量变化,透射电子显微镜观察大鼠结肠胶质细胞的超显微结构,采用酶联免疫吸附测定法检测大鼠血浆中促炎症因子白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)的水平变化。结果与模型组相比,摩腹力学刺激能明显延长玻璃小球排出时间[(2.5±0.2)min比(1.6±0.2)min,P<0.05];升高大鼠腹部抬起容量阈值[(0.5±0.1)ml比(0.4±0.1)ml,P<0.05];明显改善结肠组织病理状态;减少结肠组织中的肥大细胞数量[(2.64±0.22)个/视野比(5.61±0.12)个/视野,P<0.01];减少结肠胶质细胞中的线粒体数目和升高异染色质密度;还能减少促炎症因子IL-6[(189.4±4.7)pg/ml比(224.8±8.6)pg/ml]和IL-1β[(178.4±7.1)pg/ml比(191.4±8.4)pg/ml]的分泌,差异均具有统计学意义(均P<0.05)。结论摩腹力学刺激能提高IBS-D大鼠的内脏敏感性,调节肠胶质细胞和肥大细胞,并减少促炎症因子的分泌,其作用机制可能与肠-脑轴有关。Objective To observe the effect of abdominal mechanical stimulation on rats with irritable bowel syndrome-Diarrehea(IBS-D),and to explore the possible mechanism of abdominal massage mechanical stimulation to improve IBS-D.Method 60 newborn Wistar rats were randomly divided into blank group,model group and abdominal massage group,20 rats for each group.The IBS-D rat model was established by separating the mother rats and newborn rats.In the abdominal massage group,the IBS-D rats were treated with abdominal rubbing once a day for 14 days.In the model group,the IBS-D rats were only bound to the experimental platform once a day for 5 min for 14 days.The rats in blank group were not made model and with no intervention.The intestinal motility and visceral sensitivity of rats were measured by the time of glass bulb expulsion and the abdominal lift volume threshold.The pathological changes of rat colon tissue were observed by hematoxylin-eosin staining.The changes of mast cells in colon tissue were observed by toluidine blue staining.The ultramicrostructure of intestinal glial cells was observed by transmission electron microscope.The content of proinflammatory factors IL-6 and IL-1βin rat plasma was determined by ELISA.Results Compared with the model group,mechanical stimulation of the abdominal massage can significantly prolong the discharge time of the glass beads[(2.5±0.2)min vs(1.6±0.2)min],increase the abdominal lift volume threshold of rats[(0.5±0.1)ml vs(0.4±0.1)ml],improve the pathological state of the colon tissue,and reduce the number of mast cells[(2.64±0.22)per field vs(5.61±0.12)per field],reduce the number of mitochondria in the intestinal glial cells and increase the density of heterochromatin,and can also reduce the secretion of proinflammatory factors IL-6[(189.4±4.7)pg/ml vs(224.8±8.6)pg/ml]and IL-1β[(178.4±7.1)pg/ml vs(191.4±8.4)pg/ml],and the differences were statistically significant(all P<0.05).Conclusions Mechanical stimulation of abdominal massage can increase the visceral sensitivity

关 键 词：肠易激综合征 摩腹法 力学刺激 肠胶质细胞 促炎症因子 

分 类 号：R574.4[医药卫生—消化系统]