日粮添加谷氨酰胺对黔东南小香鸡免疫器官指数及肠道免疫的影响  

Effects of Glutamine Supplementation on the Immune Organ Indexes and Intestinal Immunity of Qiandongnan Xiaoxiang Chickens

作　　者：杨乾 张柏林[2] 刘宁[2] 孙泽威 朱玲 李家惠 陆定方 YANG Qian;ZHANG Bolin;LIU Ning;SUN Zewei;ZHU Ling;LI Jiahui;LU Dingfang(College of Animal Science and Technology,Jilin Agricultural University,Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science,Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Changchun 130118,China;College of Biology and Agriculture,Zunyi Normal University,Key Laboratory of Conservation and Utilization of Characteristic Animal Resources in Chishui River Basin,Zunyi 563006,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林省动物营养与饲料科学重点实验室,动物生产及产品质量安全教育部重点实验室,吉林长春130118 [2]遵义师范学院生物与农业科技学院,赤水河流域动物资源保护与利用重点实验室,贵州遵义563006

出　　处：《云南农业大学学报（自然科学版）》2020年第6期983-989,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：贵州省科技厅项目(黔科合基础2017[1205]);贵州省高层次创新型人才(遵市科合人才[2017]10号);遵义市校联合基金(遵市科合计[2018]08号);遵义市“15851”人才项目(2050020213#);贵州省教育厅项目(黔教合KY字[2016]258);贵州省服务农村产业革命项目(遵师合农村产业字201905)。

摘　　要：【目的】探究日粮添加谷氨酰胺(glutamine,Gln)对黔东南小香鸡免疫器官指数及肠道免疫的影响。【方法】选取90羽体质量相近、健康状况良好的1日龄黔东南小香鸡,随机分为3组,每组5个重复,每个重复6羽。对照组饲喂基础日粮,试验组在饲喂基础日粮中分别添加0.5%和1.0%的Gln。饲养至42 d,每组随机选取10羽屠宰,采集血清,分离免疫器官,刮取空肠肠黏膜,用于后续测定。【结果】与对照组相比,0.5%Gln组黔东南小香鸡脾脏指数显著升高(P<0.05),1.0%Gln组的法氏囊指数、脾脏指数、胸腺指数、血清IgA、IgG和IgM含量及空肠黏膜sIgA分泌均显著升高(P<0.05),并且空肠黏膜的MUC1和MUC2 mRNA的相对表达量也显著增加(P<0.05)。【结论】黔东南小香鸡日粮添加1.0%Gln可促进免疫器官发育,增强肠道黏膜免疫功能。[Purpose]To investigate the effects of glutamine(Gln)supplementation on immune organ index and intestinal immunity of Qiandongnan Xiaoxiang chickens.[Method]A total of 90 one-day-old Qiandongnan Xiaoxiang chickens with good health and similar initial body weight were randomly allotted to 3 groups,each group with 5 replicates and 6 chickens per replicate.Chickens in control group were fed with the basal diet,and chickens in experimental groups were supplemented with 0.5%and 1.0%Gln in the basal diets,respectively.After feeding for 42 days,10 chickens were randomly selected from each group and euthanized by cervical dislocation for serum collection.Immune organs were moved and jejunal mucosas were collected for subsequent analysis.[Result]Compared with control group,0.5%Gln supplementation significantly increased the index of spleen of Qiandongnan Xiaoxiang chicken(P<0.05).However,the spleen index,bursa index and thymus index and the contents of IgG,IgM and IgA in serum,combined with sIgA secretin in jejunum mucosa of broilers fed with 1.0%Gln were significantly increased(P<0.05).Meanwhile,the mRNA relative expressions levels of MUC1 and MUC2 in jejunum mucosa were also significantly increased(P<0.05).[Conclusion]1.0%Gln supplementation can promote the development of immune organs and strengthen the immune function of intestinal mucosa of Qiandongnan Xiaoxiang chicken.

关 键 词：谷氨酰胺 黔东南小香鸡 免疫器官指数 肠道免疫 

分 类 号：S831.4[农业科学—畜牧学]