爱康方含药血清对人肺鳞癌SK-MES-1细胞caspase-9和Bad表达水平的影响  

Effect of Aikangfang containing serum on the expression levels of caspase-9 and Bad in human lung squamous cell carcinoma SK-MES-1 cells

作　　者：马科[1] 夏淑敏 陈丽军[2] 林莹 马治国 周丽萍[2] 李巧玲 伏柏浓 田亚佳 杜海霞 马璐 MA Ke;XIA Shumin;CHEN Lijun;LIN Yin;MA Zhiguo;ZHOU Liping;LI Qiaoling;FU Bonong;Tian Yajia;DU Haixia;MA Lu(School of Traditional Chinese Medicine,Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region 750001,China;Department of Endocrinology,General Hospital of Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region 750004, China;Department of Diabetes,Yinchuan Hospital of Traditional Chinese Medicine,Yinchuan,Ningxia Hui Autonomous Region 750001,China;Department of Oncology,Mingde Hospital of Yinchuan,Yinchuan,Ningxia Hui Autonomous Region 750001,China)

机构地区：[1]宁夏医科大学中医学院,银川750001 [2]宁夏医科大学总医院内分泌科,银川750004 [3]银川市中医医院糖尿病科,750001 [4]银川市明德医院肿瘤科,750001

出　　处：《重庆医学》2020年第22期3698-3701,3708,共5页Chongqing medicine

基　　金：国家自然科学基金项目(81660829)。

摘　　要：目的观察爱康方(AKF)含药血清对人肺鳞癌SK-MES-1细胞半胱氨酸天冬氨酸蛋白酶-9(caspase-9)、Bad mRNA及蛋白表达的影响。方法将60只SD大鼠随机分为生理盐水(NS)组、AKF组、环磷酰胺(CTX)组和AKF联合CTX(AKF+CTX)组,每组15只。参照本研究课题组前期采用方法制备含药血清,各实验组(AKF组、CTX组和AKF+CTX组)以20%最佳含药血清干预人肺鳞癌SK-MES-1细胞。空白对照组细胞仅用DEME完全培养液培养。干预24 h后,采用实时荧光定量PCR(RT-qPCR)法检测caspase-9及Bad mRNA表达水平;采用Western blot检测caspase-9及Bad蛋白表达水平。结果与NS组相比,AKF组、CTX组和AKF+CTX组caspase-9、Bad mRNA及蛋白表达水平均升高,差异有统计学意义(P<0.05);各实验组组间比较,AKF+CTX组caspase-9、Bad mRNA及蛋白表达水平均明显高于CTX组及AKF组(P<0.05),CTX组caspase-9、Bad mRNA及蛋白表达水平均明显高于AKF组(P<0.05)。结论AKF促人肺鳞癌SK-MES-1细胞凋亡可能与调节caspase-9及Bad表达有关。Objective To observe the effect of Aikangfang(AKF)-containing serum on the expression of caspase-9,Bad mRNA and protein in human lung squamous cell carcinoma SK-MES-1 cells.Methods A total of 60 SD rats were randomly divided into the normal saline(NS)group,the AKF group,the cyclophosphamide(CTX)group and the AKF combined with CTX(AKF+CTX)group,with 15 rats in each group.The medicated serum was prepared according to the method used in the early stage of this research group.The human lung squamous cell carcinoma SK-MES-1 cells in all experimental groups,including the AKF group,the CTX group and the AKF+CTX group,were intervened by 20%optimal medicated serum.The cells of the blank control were only cultured with DEME complete culture medium.After 24 hours of intervention,real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the expression levels of caspase-9 and Bad mRNA,and Western blot was used to detect the expression levels of caspase-9 and Bad protein.Results Compared with the NS group,the expression levels of caspase-9,Bad mRNA and protein increased in the AKF group,the CTX group and the AKF+CTX group,and the differences were statistically significant(P<0.05).Among the experimental groups,the expression levels of caspase-9,Bad mRNA and protein in the AKF+CTX group were significantly higher than those in the CTX group and the AKF group(P<0.05),and the expression levels of caspase-9,Bad mRNA and protein in the CTX group were significantly higher than those in the AKF group(P<0.05).Conclusion AKF promotes apoptosis of human lung squamous cell carcinoma SK-MES-1 cells,which may be related to the regulation of caspase-9 and Bad expression.

关 键 词：肺肿瘤 爱康方 含药血清 肿瘤 鳞状细胞 半胱氨酸天冬氨酸蛋白酶9 BAD 

分 类 号：R734.2[医药卫生—肿瘤]