CD74介导巨噬细胞移动抑制因子对宫颈癌细胞生物学行为的影响  

Effect of macrophage migration inhibitory factor mediated by CD74on regulating biological behavior of cervical cancer cells

作　　者：成荣杰[1] 李楠[2] CHENG Rong-jie;LI Nan(Department of Gynecology and Obstetrics,The Fourth Affiliated Hospital of Harbin Medical University,Harbin150001,China)

机构地区：[1]哈尔滨医科大学附属第四医院妇产科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第四医院病理科,黑龙江哈尔滨150001

出　　处：《中国实验诊断学》2020年第10期1689-1693,共5页Chinese Journal of Laboratory Diagnosis

基　　金：黑龙江省自然科学青年基金资助项目(QC2018100);黑龙江省博士后基金(LBH-Z18116)。

摘　　要：目的观察巨噬细胞移动抑制因子(MIF)及其受体CD74对宫颈鳞癌细胞株SiHa增殖、侵袭、转移等生物学行为的影响。方法培养人宫颈癌SiHa细胞,免疫细胞化学法检测细胞中MIF及CD74蛋白的表达情况;应用外源性人重组MIF(rhMIF)、抗-CD74单抗处理细胞,分别采用CCK-8法、细胞划痕实验及Transwell小室法观察MIF及CD74对癌细胞增殖、迁移及侵袭力的改变。结果免疫细胞化学法显示MIF及CD74蛋白均可在SiHa细胞中表达;rhMIF处理后细胞增殖力加强、呈时间-剂量依赖性,且细胞的体外迁移及侵袭力明显提升(P<0.05);当用抗CD74单抗预处理细胞后,可抑制MIF的促宫颈癌细胞恶性增殖作用,但对细胞的迁移及侵袭能力无明显影响。结论MIF具有调控SiHa细胞增殖、迁移及侵袭等生物学行为的作用,其中CD74介导MIF促进了癌细胞的恶性增殖力,提示两者在宫颈癌的发生发展中起重要作用。Objective To investigate the proliferation,migration and invasion of human cervical cancer SiHa cells induced by MIF and CD74.Methods Immunocytochemistry was used to detect the expression of MIF and CD74proteins in cervical cancer SiHa cells;The SiHa cells treated with rhMIF,and anti-CD74monoclonal antibody were used to observe the changes in the proliferation,migration and invasion by CCK-8kit、Cell scratch method and Boyden chamber respectively.Results Immunocytochemistry detected that MIF and CD74proteins were expressed in SiHa cells;The Si-Ha cells proliferation,which was increased treated with exogenous rhMIF in dose-time dependent manner,and the effect of could be partially inhibited with anti-CD74monoclonal antibody;rhMIF increased the migration and invasion of SiHa cell in vitro.However,CD74don’t take part in the migratory and metastatic courses.Conclusion All the results indicated that MIF can promote the biological behaviors,such as proliferation,migration and increased the invasion potency of cervical cancers,Among them,CD74mediated MIF promoted the malignant proliferation of cancer cells,suggesting that they play an important role in the occurrence and development of cervical cancer.

关 键 词：巨噬细胞移动抑制因子(MIF) 宫颈癌 CD74 增殖 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]