肺鳞癌组织miRNA-193a及miRNA-4286水平与预后的关系  

Relationship between the levels of miRNA-193a and miRNA-4286 in tissues of squamous cell lung carcinoma and prognosis

作　　者：毕锐[1] 王明松[1] 胡睿[1] 蒋连勇[1] 杨旭辉 钟元 BI Rui;WANG Mingsong;HU Rui;JIANG Lianyong;YANG Xuhui;ZHONG Yuan(Department of Cardiothoracic Surgery,Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai200092,China)

机构地区：[1]上海交通大学医学院附属新华医院心胸外科,上海200092

出　　处：《中国医药导报》2020年第33期9-12,60,共5页China Medical Herald

基　　金：国家自然科学基金面上项目(81772459)。

摘　　要：目的探讨肺鳞癌患者肿瘤组织中微小RNA(miRNA)-193a和miRNA-4286的表达水平与预后的关系,为肺癌预后评价提供新方向。方法选取2016年5月—2019年4月上海交通大学医学院附属新华医院确诊且临床资料完整的126例肺鳞癌患者为研究对象,通过实时荧光定量逆转录PCR(qRT-PCR)检测肺鳞癌组织和癌旁组织miRNA-193a和miRNA-4286的表达水平,分析肺鳞癌组织miRNA-193a和miRNA-4286与临床资料和预后的关系。结果肺鳞癌组织中miRNA-193a相对表达量低于癌旁组织,miRNA-4286相对表达量高于癌旁组织,差异均有统计学意义(均P<0.05);肺鳞癌组织内miRNA-193a和miRNA-4286水平与年龄、性别、吸烟史和家族史无关联,差异无统计学意义(P>0.05),与肿瘤大小、TNM分期、淋巴结转移和复发情况有关联,差异均有统计学意义(均P<0.05);肺鳞癌组织内miRNA-193a与分化程度无关联(P>0.05),而miRNA-4286与分化程度有关联(P<0.05)。肺鳞癌组织miRNA-193a低表达患者的中位生存期为36个月(95%CI:0.371~1.198),而高表达患者为54个月(95%CI:0.834~2.696),差异有统计学意义(P<0.05);肺鳞癌组织miRNA-4286低表达患者的中位生存期为51个月(95%CI:0.962~2.660),而高表达患者为39个月(95%CI:0.376~1.039),差异有统计学意义(P<0.05)。结论肺鳞癌组织miRNA-193a和miRNA-4286表达水平与肿瘤大小、TNM分期、淋巴结转移和复发情况有关联,且与患者预后有关,miRNA-193a和miRNA-4286可能成为评价肺鳞癌患者预后的指标。Objective To explore the relationship between the expression levels of microRNA(miRNA)-193a and miRNA-4286 in the tumor tissues of squamous cell lung carcinoma patients and prognosis,so as to provide a new direction for the prognosis evaluation of lung cancer.Methods A total of 126 patients with squamous cell lung carcinoma diagnosed from May 2016 to April 2019 with complete clinical data in Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine were selected as research objects.The expression levels of miRNA-193a and miRNA-4286 in squamous cell lung carcinoma tissues and adjacent tissues were detected by real-time fluorescent quantitative reverse transcription PCR(qRT-PCR),and the relationship among miRNA-193a and miRNA-4286 in squamous cell lung carcinoma tissues and clinical data and prognosis were analyzed.Results The relative expression level of miRNA-193a in squamous cell lung carcinoma tissues was lower than that in adjacent tissues,and the relative expression level of miRNA-4286 was higher than that in adjacent tissues,with statistically significant differences(all P<0.05).The levels of miRNA-193a and miRNA-4286 in squamous cell lung carcinoma tissues were not correlated with age,sex,smoking history or family history,and the differences were not statistically significant(P>0.05).They were correlated with tumor size,TNM stage,lymph node metastasis and recurrence,and the differences were statistically significant(all P<0.05).miRNA-193a in squamous cell lung carcinoma tissues was not associated with the degree of differentiation(P>0.05),while miRNA-4286 was associated with the degree of differentiation(P<0.05).The median survival time of patients with low miRNA-193a expression in squamous cell lung carcinoma tissues was 36 months(95%CI:0.371-1.198),while that of patients with high expression was 54 months(95%CI:0.834-2.696),the difference was statistically significant(P<0.05).The median survival time of patients with low miRNA-4286 expression in squamous cell lung carcinoma tissues wa

关 键 词：微小RNA-193a 微小RNA-4286 肺鳞癌 预后 

分 类 号：R734.2[医药卫生—肿瘤]