糖芯片技术在肿瘤研究中的应用  

Application of glycan microarrays in cancer research

作　　者：许倍宁 刘银坤 谢裕安[1] Beining Xu;Yinkun Liu;Yu’an Xie(Department of Oncology Research,Guangxi Medical University Affiliated Tumor Hospital&Oncology Medical College,Nanning 530021,Guangxi,China;Key Laboratory of Carcinogenesis and Cancer Invasion(Ministry of Education),Liver Cancer Institute,Zhongshan Hospital,Fudan University,Shanghai 200032,China)

机构地区：[1]广西医科大学附属肿瘤医院实验研究部,广西南宁530021 [2]复旦大学附属中山医院肝癌研究所癌变与侵袭原理教育部重点实验室,上海200032

出　　处：《生物工程学报》2020年第11期2313-2326,共14页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.81260320)资助。

摘　　要：糖基化修饰是蛋白质常见的翻译后修饰之一,通过与糖结合蛋白如凝集素、抗体等相互作用调节肿瘤细胞侵袭、转移的能力及肿瘤异质性。通过化学合成法、化学-酶合成法或释放天然聚糖构建的糖芯片是分析聚糖与糖结合蛋白相互作用的重要工具。文中综述了常见的点制糖芯片的技术及糖芯片在癌症疫苗、单克隆抗体及诊断标志物中的广泛运用。由于肿瘤发生的各个环节都伴随着聚糖结构的改变,利用糖芯片探究肿瘤细胞特异表达的聚糖所参与的生理病理过程具有重大意义。Glycosylation is one of the common post-translational modifications of proteins to regulate the ability of tumor invasion,metastasis and tumor heterogeneity by interacting with glycan-binding proteins such as lectins and antibodies.Glycan microarray can be constructed by chemical synthesis,chemical-enzyme synthesis or natural glycan releasing.Glycan microarray is an essential analytical tool to discover the interaction between glycan and its binding proteins.Here we summarize the standard techniques to construct glycan microarray for the application in cancer vaccine,monoclonal antibody and diagnostic markers.

关 键 词：糖芯片 糖结合蛋白 癌症疫苗 单克隆抗体 诊断标志物 

分 类 号：R73-3[医药卫生—肿瘤]