长链非编码RNA淋巴细胞白血病缺失基因1在食管鳞癌中的表达及其对细胞增殖、迁移和侵袭的影响  

Expression of long non-coding RNA DLEU1 in esophageal squamous cell carcinoma and its effect on cell proliferation,migration and invasion

作　　者：顾术东[1] 缪捷飞[1] 沈洪[1] 张曙[2] 茅国新[1] GU Shudong;MIAO Jiefei;SHEN Hong;ZHANG Shu;MAO Guoxin(Department of Oncology,Affiliated Hospital of Nantong University,Nantong 226001,China;不详)

机构地区：[1]南通大学附属医院肿瘤科,江苏南通226001 [2]南通大学附属医院病理科,江苏南通226001

出　　处：《实用医学杂志》2020年第21期2911-2915,共5页The Journal of Practical Medicine

基　　金：南通市科技局项目(编号:JC2018154)。

摘　　要：目的分析长链非编码RNA(long non-coding RNA,lncRNA)淋巴细胞白血病缺失基因1(deleted in lymphocytic leukemia 1,DLEU1)在食管鳞癌(esophageal squamous cell carcinoma,ESCC)中的表达及意义,并进一步研究DLEU1对ESCC细胞TE-13增殖、迁移和侵袭的影响。方法采用实时荧光定量PCR检测ESCC组织和癌旁组织中DLEU1的表达。通过CCK-8、划痕试验和Transwell侵袭试验分析DLEU1表达对TE-13增殖、迁移和侵袭的影响。结果DLEU1在ESCC癌组织中的表达较癌旁组织明显升高(P<0.05)。DLEU1的表达与淋巴结转移和TNM分期相关(P<0.05),而与患者的年龄、性别、分化程度和浸润深度均无关(P>0.05)。生存分析示,DLEU1高表达ESCC患者的生存时间较低表达患者明显缩短(P<0.05)。干扰DLEU1表达后TE-13的增殖、迁移和侵袭能力显著受抑。结论DLEU1在ESCC中表达上调,DLEU1的高表达提示预后不良,DLEU1可能对ESCC细胞TE-13的增殖、迁移和侵袭具有调节作用,DLEU1有可能成为ESCC治疗的潜在靶点。Objective To analyze the expression of long-chain non-coding RNA(lncRNA)lymphocyte leukemia deletion gene 1(DLEU1)in esophageal squamous cell carcinoma(ESCC)and its clinical significance.The effect of DLEU1 on the proliferation,migration and invasion of TE-13 was further studied.Methods Real-time quantitative polymerase chain reaction was used to detect the expression of DLEU1 in ESCC and adjacent normal tissues.The effects of DLEU1 on the proliferation,migration and invasion of TE-13 were analyzed by CCK-8,scratch test and Transwell test.Results The expression of DLEU1 in ESCC was significantly higher than that in adjacent normal tissues(P<0.05).The expression of DLEU1 was correlated with lymph node metastasis and TNM stage(P<0.05),but not with age,gender,degree of differentiation and depth of invasion(P>0.05).After knockdown of DLEU1 in TE-13 cells,cell proliferation,migration and invasion were significantly suppressed.Conclusion The expression of DLEU1 was up-regulated in ESCC,and the high expression of DLEU1 suggested a poor prognosis.DLEU1 may have a regulatory effect on the proliferation,migration and invasion of TE-13 cells,and it may become a potential target for ESCC treatment.

关 键 词：淋巴细胞白血病缺失基因1 食管鳞癌 增殖 迁移 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]