TIAM1下调表达对食管癌细胞恶性增殖和迁移的影响  

Suppression of TIAM1 Inhibits Malignant Proliferation and Migration of Esophageal Carcinoma Cell

作　　者：俞佳鑫 吴一航 陈威[1] 陈文虎[1] 王黎芳[1] 赵宏光[2] YU Jiaxin;WU Yihang;CHEN Wei;CHEN Wenhu;WANG Lifang;ZHAO Hongguang(Hangzhou Medical College,Hangzhou 310053,China;Zhejiang Cancer Hospital,Hangzhou 310022,China)

机构地区：[1]杭州医学院,浙江杭州310053 [2]浙江省肿瘤医院,浙江杭州310022

出　　处：《浙江医学教育》2020年第6期44-47,共4页Zhejiang Medical Education

基　　金：浙江省自然科学基金(编号:LY18H160036);浙江省教育厅一般项目(编号:Y201738529);浙江省新苗人才项目(编号:2019R425008)。

摘　　要：目的:探讨TIAM1下调表达对食管癌(ESCA)细胞恶性增殖和迁移的影响。方法:采用免疫组织化学方法和RT-qPCR检测TIAM1在食管鳞癌临床组织中的表达情况;使用慢病毒介导的小干扰RNA构建TIAM1低表达的Eca109细胞株;CCK-8试验和集落形成测定评估TIAM1的表达对细胞增殖的影响。细胞划痕实验检测TIAM1对细胞迁移活性的影响;结果:相比于癌旁正常组织,ESCA组织中检测到的TIAM1表达水平更高(P<0.05)。下调TIAM1表达抑制了细胞增殖和集落形成(P<0.05),降低了细胞的迁移能力。结论:TIAM1在ESCA细胞的增殖和迁移中起着至关重要的作用,提示TIAM1可作为ESCA诊断及治疗中的潜在生物标志物。[Objective]To explore the effect of TIAM1 down-regulationon the malignant proliferation and migration of esophageal cancer cells.[Method]The expression patterns of TIAM1 in ESCA clinical tissues were evaluated using immunohistochemistry and RT-qPCR methods.Lentiviral-mediated small interfering RNAs were used to knockdown TIAM1 in Eca109 cell.CCK-8 and colony formation assays were used to assess the effect of TIAM1 on cell proliferation.The effect of TIAM1 on cell migratory activity was detected by cell scratch experiment.[Result]Higher level of TIAM1 expression was detected ESCA tissue compared to adjacent non-cancerous epithelium.Knockdown of TIAM1 expression was not only suppressed cell proliferation in CCK8 assay,colony formation assay(P<0.05),but also inhibited the cell migratory capacity in vitro(P<0.05).[Conclusion]TIAM1 played a critical role in ESCA cells proliferation and migration,suggesting TIAM1 to be a potential biomarker for the ESCA therapy.

关 键 词：T淋巴瘤侵袭转移诱导蛋白1 食管癌 细胞增殖 细胞迁移 

分 类 号：R735.1[医药卫生—肿瘤]