饲料中添加戊糖乳杆菌HC-2对黄曲霉毒素B1刺激下的凡纳对虾肝胰腺显微结构、基因表达及酶活性的影响  

Effects of Lactobacillus pentosus HC-2 on the morphology of hepatopancreas,immunity-related genes,and enzyme activity of Penaeus vannamei affected by aflatoxin B1

作　　者：房涵 刘梅[1,2,4] 王宝杰 蒋克勇[1,2,4] 王雷 FANG Han;LIU Mei;WANG Bao-jie;JIANG Ke-yong;WANG Lei(CAS Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China;University of Chinese Academy of Sciences,Beijing 100049,China;Center for Ocean Mega-Science,Chinese Academy of Sciences,Qingdao 266071,China)

机构地区：[1]中国科学院海洋研究所中国科学院实验海洋生物学重点实验室,山东青岛266071 [2]青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室,山东青岛266237 [3]中国科学院大学,北京100049 [4]中国科学院海洋大科学中心,山东青岛266071

出　　处：《海洋科学》2020年第11期65-71,共7页Marine Sciences

基　　金：国家重点研发计划课题(2019YFD0900401);山东省重大科技创新工程专项课题(2018SDKKJ0502-2)。

摘　　要：为探究饲料中添加戊糖乳杆菌HC-2对黄曲霉毒素B1(AFB1)刺激下的凡纳对虾肝胰腺显微结构、基因表达及肝胰腺酶活性的影响,将健康的凡纳对虾(900尾)随机分成三组,分别投喂基础饲料、添加AFB1(500μg/kg)饲料以及添加AFB1(500μg/kg)+戊糖乳杆菌HC-2(5×10^8 CFU/g)饲料6周。在试验结束后,分别取三组对虾的肝胰腺进行显微观察以及免疫相关基因和肝胰腺酶活性的测定。AFB1+HC-2组肝胰腺组织同AFB1组相比损伤程度较轻。同对照组相比,AFB1组和AFB1+HC-2组的肝胰腺免疫基因Rab、GST、mucin-like PM、Dorsal、Relish、Pro-PO的相对表达量均呈现显著下调(P<0.05),且同AFB1组相比,AFB1+HC-2组的免疫相关基因GST、Dorsal、Pro-Po的相对表达量下调的较少(P<0.05)。同对照组相比,AFB1组和AFB1+HC-2组的碱性磷酸酶活性(AKP)明显上升,且AFB1+HC-2组的AKP活性要高于AFB1组。同对照组相比,AFB1组和AFB1+HC-2组中谷胱甘肽巯基转移酶(GST)活性明显升高但两组间无显著性差异,超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性在AFB1组和AFB1+HC-2组间无显著性差异,且三组的总抗氧化能力(T-AOC)无显著性差异。研究认为饲料中添加戊糖乳杆菌HC-2对AFB1刺激下的凡纳对虾肝胰腺组织结构有一定程度的保护作用,对部分免疫基因和碱性磷酸酶活性有显著影响,但对肝胰腺总抗氧化能力没有显著影响。To explore effects of Lactobacillus pentosus HC-2 on the hepatopancreas morphology,immune-related genes,and enzyme activity of Penaeus vannamei affected by aflatoxin B1(AFB1),shrimps were fed with formulated feeds containing AFB1(500μg/kg),AFB1(500μg/kg)+L.pentosus HC-2(5×10^8 CFU/g),and a basal commercial diet(control group)respectively.At the end of the experiment,the morphology of the hepatopancreas,expression of immune-related genes,and activity of hepatopancreas enzymes were detected.The hepatopancreas morphology of the AFB1+L.pentosus HC-2 group suffered less damage than that of the AFB1 group.The relative expression of immune-related genes(Rab,glutathione S-transferase[GST],mucin-like PM,Dorsal,Relish,and Pro–PO)in the AFB1 and AFB1+HC-2 groups was significantly lower than in the control group(P<0.05),and the relative expression of immune-related genes(GST,Dorsal,and Pro–PO)in the AFB1+HC-2 group was higher than in the AFB1 group(P<0.05).The activity of alkaline phosphatase(AKP)increased significantly in the AFB1 and AFB1+HC-2 groups compared with that in the control group,and the activity of AKP in the AFB1+HC-2 group was higher than in the AFB1 group.The activity of GST increased in the AFB1 and AFB1+HC-2 groups compared with that in the control group,and there was no significant difference in the AFB1+HC-2 and AFB1 groups.The activity of superoxide dismutase and catalase has no significant difference between the AFB1 and AFB1+HC-2 groups,and the total antioxidant capacity(T-AOC)has no significant difference between the control and experimental groups.We concluded that L.pentosus HC-2 had a positive effect on the hepatopancreas structure of shrimps stimulated by AFB1 and induced a significant effect on some immune genes and AKP activity but had no significant effect on the T-AOC of the hepatopancreas.

关 键 词：凡纳对虾 戊糖乳杆菌HC-2 黄曲霉毒素B1 基因表达 肝胰腺酶活 

分 类 号：S968.22[农业科学—水产养殖]