肌肉生长抑制素基因(MSTN)编辑牛的肉质相关蛋白质组学研究  

Proteomics Study on Meat Quality of Myostatin(MSTN)Gene-edited Cattle(Bos taurus)

作　　者：李欣 海超 刘春丽 白春玲 魏著英 李光鹏 赵跃芳 LI Xin;HAI Chao;LIU Chun-Li;BAI Chun-Ling;WEI Zhu-Ying;LI Guang-Peng;ZHAO Yue-Fang(State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock,Inner Mongolia University,Hohhot 010070,China)

机构地区：[1]内蒙古大学,省部共建草原家畜生殖调控与繁育国家重点实验室,呼和浩特010070

出　　处：《农业生物技术学报》2020年第11期1970-1984,共15页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31560333);内蒙古自治区自然科学基金(2017MS0318);内蒙古自治区科技厅重大专项省部共建草原家畜生殖调控与繁育国家重点实验室自主课题(zdzx2018065);内蒙古自治区科技创新引导项目(KCBJ2018002)。

摘　　要：哺乳动物中肌肉生长抑制素基因(myostatin,MSTN)功能缺失会使肌肉过度发育,生产性能显著提高,同时对肉质性状也具有一定的影响。本研究以非标定量蛋白质组学技术研究MSTN基因编辑牛(Bos taurus)(简称Exp)与野生型(简称Ctr)的肌肉差异蛋白质表达谱,共鉴定到有定量值的蛋白质1595个,其中上调表达的蛋白有28个(Exp/Ctr>1.5,P<0.05),下调表达的蛋白有132个(Exp/Ctr<0.67,P<0.05)。这些差异表达蛋白主要参与肌纤维组成、脂代谢调控、肌肉收缩、氧化呼吸电子传递等。差异表达蛋白中,已报到的肉质相关蛋白有27个,其中肌纤维相关蛋白有肌球蛋白重链(myosin heavy chain,MYH)1、MYH6、肌球蛋白轻链6(myosin light polypeptide 6,MYL6)、肌球蛋白结合蛋白H(myosin binding protein H,MYBPH)和肌球蛋白2(myomesin(M-protein)2,MYOM2);脂肪合成相关蛋白有脂联素(adiponectin,ADIPOQ)、钙粘蛋白13(cadherin-13,CDH13)、烯酰辅酶A水合酶1(enoyl coenzyme A hydratase 1,ECH1)、过氧化氢酶(catalase,CAT)和波形蛋白(vimentin,VIM),上述蛋白表达均下调;线粒体NADH(nicotinamide adenine dinucleotide)脱氢酶的9个蛋白亚基也表达下调。通过STRING数据库进行蛋白质相互作用分析发现,上述蛋白与MSTN具有潜在的相互作用,其中MYH6、MYH1、ADIPOQ与MSTN直接互作。本研究从蛋白质组层面揭示了MSTN基因可能通过改变肌纤维组成、影响肌内脂肪合成以及肌肉供能方式而影响牛肉品质,对肉品质调控的分子机制研究具有一定的指导意义。The myostatin gene(MSTN)is a negative regulator of skeletal muscle development,and its function lose in mammals leads to overdevelopment of muscle,significant improvement of production performance,and certain effects on meat quality traits.The present study utilized the muscle tissues from MSTN gene edited Luxi beef cattle(Bos taurus)(Exp for short)and wild type(Ctr for short)to perform label free quantitative comparative proteomics,and investigate the effect of MSTN artificial mutation on meat quality traits.A total of 1595 quantified proteins containing at least one unique peptide per protein were identified.After scatter plotting analysis used for determining the internal error of the biological replicates and student t-test analysis of the data set,the proteins with fold change of Exp/Ctr in relative abundance more than 1.5 or less than 0.67 and a P-value less than 0.05 from t-test were identified as differentially expressed proteins(DEPs).As a result,160 DEPs were identified which contained 28 proteins in increased abundance and 132 in decreased abundance.Gene Ontology(GO)enrichment and KEGG pathway analysis were performed to explore molecular mechanism of DEPs in the determination of meat quality.There were 5 GO terms of biological process,7 of cellular component,and 5 of molecular function enriched significantly.The biological process were related to oxidation reduction process,skeletal muscle contraction,transition between fast and slow fiber,mitochondrial electron transport nicotinamide adenine dinucleotide(NADH)to ubiquinone,cardiac muscle hypertrophy in response to stress.The cellular component were extracellular exosome,mitochondrial respiratory chain complex Ⅰ,mitochondrion,Z disc,focal adhesion,extracellular matrix,mitochondrial inner membrane.The molecular function were actin binding,NADH dehydrogenase(ubiquinone)activity,protein C-terminus binding,structural constituent of muscle,thioredoxin peroxidase activity.KEGG pathway analysis results included non-alcoholic fatty liver disease(NAFLD),oxida

关 键 词：肌肉生长抑制素(MSTN)基因编辑牛 蛋白质组学 肉质 肌内脂肪 

分 类 号：S823.92[农业科学—畜牧学]