黄芪提取物对乳腺癌细胞增殖的抑制作用机制  

Inhibition Mechanism of Astragalus membranaceus Extract on Proliferation of Breast Cancer Cells

作　　者：宋凯[1] Song Kai(Xuzhou Vocational College of Bioengineering,Xuzhou,221006)

机构地区：[1]徐州生物工程职业技术学院,徐州221006

出　　处：《基因组学与应用生物学》2020年第8期3792-3796,共5页Genomics and Applied Biology

摘　　要：黄芪是中药中常用的中草药。本研究旨在探讨黄芪提取物对乳腺癌细胞的生物学作用及其机制。将干燥的黄芪粉碎,采用水提-乙醇上清法提取,制备提取液。通过MTT比色法和形态学观察,检测黄芪提取物的抗增殖活性;以流式细胞仪检测细胞凋亡情况;免疫印迹法检测细胞总PI3K、磷酸化PI3K、GS3Kβ、AKT和mTOR的表达。MTT实验和形态学观察表明,黄芪提取物对MCF-7、SK-BR-3和MDA-MB-231细胞的增殖均有抑制作用,且呈剂量依赖性。流式细胞仪分析显示,经20μg/mL和40μg/mL黄芪提取物处理后,乳腺癌细胞凋亡率明显高于二甲基亚砜和空白对照组(p<0.05)。Western blotting分析发现,与二甲基亚砜对照组相比,pPI3K、pGS3Kβ、pAKT和pmTOR水平显著降低。综上所述,黄芪提取物通过PI3K/AKT/mTOR途径抑制细胞增殖和诱导细胞凋亡,证实了黄芪的抗肿瘤作用。本研究结果为黄芪提取物作为一种有前途的乳腺癌治疗药物的抗癌作用提供了新的视角。Astragalus membranaceus is a Chinese herb commonly used in traditional Chinese medicine.The aim of this study was to investigate the biological effect of Astragalus membranaceus extract on breast cancer cells and its mechanism.The dried Astragalus membranaceus was crushed and extracted by water extraction and ethanol supernatant.The antiproliferative activity of Astragalus membranaceus extract was detected by MTT colorimetry and morphological observation.Cell apoptosis was detected by flow cytometry.The expression of total PI3K,phosphorylated PI3K,GS3K AKT and mTOR was detected by Western blotting.MTT assay and morphological observation showed that Astragalus membranaceus extract inhibited the proliferation of SK-BR-3 and MDA-MB-231 cells in a dose-dependent manner.Flow cytometry analysis showed that after 20μg/mL and 40μg/mL Astragalus membranaceus extract treatment,the apoptosis rate of breast cancer cells was significantly higher than that of dimethyl sulfoxide and blank control group(p<0.05).Western blotting analysis showed that compared with the dimethyl sulfoxide control group,pPI3K,pGS3K,pAKT and pmTOR levels were significantly decreased.In conclusion,Astragalus membranaceus extract inhibits cell proliferation and induces apoptosis through PI3K/AKT/mTOR pathway,confirming the anti-tumor effect of Astragalus membranaceus.The results of this study provide a new perspective on the anticancer effects of Astragalus membranaceus extract as a promising drug for the treatmentof breast cancer.

关 键 词：黄芪(Astragalus membranaceus) 乳腺癌 细胞凋亡 PI3K/AKT/mTOR途径 

分 类 号：R285.5[医药卫生—中药学]