Role of the periotic mesenchyme in the development of sensory cells in early mouse cochlea  

作　　者：Jingjiang Huang Na Zuo Cheng Wu Peipei Chen Jun Ma Chuanxi Wang Wenyan Li Shaofeng Liu 

机构地区：[1]Department of Otolaryngology-Head and Neck Surgery,Yijishan Hospital of Wannan Medical College,Wuhu,Anhui,241001,China [2]ENT Institute and Otorhinolaryngology Department of Affiliated Eye and ENT Hospital,State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai,200031,China [3]Key Laboratory of Hearing Medicine of NHFPC,Shanghai,200031,China

出　　处：《Journal of Otology》2020年第4期138-143,共6页中华耳科学杂志（英文版）

基　　金：the Chinese National Natural Science Foundation of China(grant number 81371089)。

摘　　要：Objective:To investigate the role of the periotic mesenchyme(POM)in the development of sensory cells of developing auditory epithelium.Methods:Developing auditory epithelium with or without periotic mesenchyme was isolated from mice at embryonic days 11.5(E11.5),E12.5 and E13.5,respectively,and cultured in vitro to an equivalent of E18.5’s epithelium in vivo.Then,the explants were co-stained with antibodies targeting myosin VIIA,Sox2 and BrdU.Results:More hair cells in E11.5 t 7 DIV,E12.5 t 6 DIV and E13.5 t 5 DIV auditory epithelia were found upon culture with POM(225.90±62.44,476.94±100.81,and 1386.60±202.38,respectively)compared with the non-POM group(68.17±23.74,205.00±44.23,and 1266.80±38.84,respectively).Moreover,regardless of developmental stage,the mesenchymal tissue increased the amount of cochlear sensory cells as well as the ratio of differentiated hair cells to total sensory cells.Conclusions:The periotic mesenchyme promotes the development of cochlear sensory cells,and its effect depends on the developmental stage of the auditory epithelium.

关 键 词：Inner ear COCHLEA Sensory cells MESENCHYME Development 

分 类 号：R764[医药卫生—耳鼻咽喉科]