超高效液相色谱-串联质谱法测定夏枯草中4种酚酸含量  

Simultaneous Determination of Four Phenolic Acids in Prunella Vulgaris by UPLC-MS/MS

作　　者：柏玉冰 李洪权 包敏 林艳[3] BAI Yubing;LI Hongquan;BAO Min;LIN Yan(The Fis Hopial of Hunan University of Chinese Medicine Changsha,Hunan,China 41007;Zhuhou Institue for Fod and Dnug Control,Zhuzhou,Hunan,China 412000;The Hunan Unitversity of Chinese Medicine,Changsha,Hunan,China 410208)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410009 [2]湖南省株洲市食品药品检验所,湖南株洲412000 [3]湖南中医药大学,湖南长沙410208

出　　处：《中国药业》2020年第23期24-27,共4页China Pharmaceuticals

基　　金：国家重点研发计划子课题项目[2017YFC1701903-8]。

摘　　要：目的建立同时测定夏枯草中绿原酸、阿魏酸、原儿茶酸、原儿茶醛4种酚酸类成分含量的超高效液相色谱-串联质谱(UPLCMS/MS)法。方法夏枯草以50%甲醇超声提取,色谱柱采用Welch Xtimate C18柱(150 mm×4.6 mm,5μm),流动相为0.1%甲酸水-乙腈(梯度洗脱),流速为0.5 m L/min,柱温为30℃,进样量为2μL,采用电喷雾离子源(ESI)。结果绿原酸、阿魏酸、原儿茶酸、原儿茶醛质量浓度分别在0.019 6~2.940 0μg/m L,0.022 5~3.375 0μg/m L,0.022 3~3.345 0μg/m L,0.027 8~4.170 0μg/m L范围内与峰面积线性关系良好;平均回收率分别为98.25%,100.89%,99.57%,98.57%,RSD分别为2.29%,1.96%,1.59%,2.11%(n=6);夏枯草样品中4种成分含量绿原酸为459.75~1 500.85μg/g,阿魏酸为120.84~195.13μg/g,原儿茶酸为204.16~669.24μg/g,原儿茶醛为77.41~315.56μg/g。结论该方法快速、准确,可用于夏枯草药材中上述4种酚酸类成分含量的测定。Objective To establish a UPLC-MS/MS method for the simultaneous determination of four phenolic acids(chlorogenic acid,ferulic acid,protocatechuic acid,protocatechualdehyde)in Prunella vulgaris.Methods Prunella vulgaris was extracted by ultrasound with50%methanol.The chromatographic column was Welch Xtimate C18 column(150 mm×4.6 mm,5μm),the mobile phase was 0.1%formic acid-acetonitrile with gradient elution,the flow rate was 0.5 m L/min,the column temperature was 30℃,and the injection volume was 2μL.The mass spectra were obtained by electron spray ionization(ESI).Results The linear ranges were 0.0196-2.9400μg/m L for chlorogenic acid,0.0225-3.3750μg/m L for ferulic acid,0.0223-3.3450μg/m L for protocatechuic acid,0.0278-4.1700μg/m L for protocatechualdehyde,the average recoveries were 98.25%,100.89%,99.57%and 98.57%,and the RSDs were2.29%,1.96%,1.59%and 2.11%(n=6),respectively.The contents of chlorogenic acid,ferulic acid,protocatechuic acid and protocatechualdehyde were 459.75-1500.85μg/g,120.84-195.13μg/g,204.16-669.24μg/g,77.41-315.56μg/g,respectively.Conclusion The method is rapid and accurate,which can be used for the content determination of the four phenolic acids in Prunella vulgaris.

关 键 词：超高效液相色谱-串联质谱法 夏枯草 酚酸 绿原酸 阿魏酸 原儿茶酸 原儿茶醛 

分 类 号：R932[医药卫生—生药学] R2841[医药卫生—药学]