水溶性磷酸盐柱[5]芳烃与吖啶橙的络合行为  

Complexation Analysis of Water-Soluble Phosphate Salt Pillar[5]arene with Acridine Orange

作　　者：保秋连 杨云汉 魏可可 罗建萍 古捷 鲁佳佳 杨丽娟 BAO Qiu-lian;YANG Yun-han;WEI Ke-ke;LUO Jian-ping;GU Jie;LU Jia-jia;YANG Li-juan(Key Laboratory of Intelligent Supramolecular Chemistry at the University of Yunnan Province,National and Local Joint Engineering Research Center for Green Preparation Technology of Biobased Materials,School of Chemistry&Environment,Yunnan Minzu University,Kunming 650500,China)

机构地区：[1]云南民族大学化学与环境学院,云南省高校智能超分子化学重点实验室,生物基材料绿色制备技术国家地方联合工程中心,云南昆明650500

出　　处：《波谱学杂志》2020年第4期469-483,共15页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目(21562048,21762051);云南省高校有机功能分子及材料科技创新团队资助项目;云南省教育厅科学研究基金资助项目(2020Y0261)。

摘　　要：本文首先构建了水溶性磷酸盐柱[5]芳烃(PP5A)与阳离子荧光染料吖啶橙(AO)主-客体络合物.然后通过紫外光谱(UV)、红外吸收光谱(IR)、分子荧光光谱(MFS)、一维和二维核磁共振(NMR)波谱技术(包括1H NMR和NOESY)研究了PP5A与AO的络合行为.此外,还研究了pH及两种表面活性剂——十二烷基硫酸钠(SDS)和十六烷基三甲基溴化铵(CTAB)对AO/PP5A络合体系的荧光强度的影响.最后,采用分子对接计算了PP5A与AO和CTAB的络合模式及络合能.研究表明,AO与PP5A形成1:1的主-客体络合物;在pH=3~11范围内,PP5A均能使AO的荧光强度发生减弱或淬灭;SDS和CTAB的加入并不会对AO/PP5A络合体系的荧光强度产生显著影响.AO与PP5A络合时产生的荧光强度变化可为荧光传感器的设计提供理论基础.The host-guest complex of water-soluble phosphate salt pillar[5]arene(PP5A)and cationic fluorescent dye acridine orange(AO)was constructed,and studied with ultraviolet spectrum(UV),infrared absorption spectrum(IR),molecular fluorescence spectroscopy(MFS),and nuclear magnetic resonance(NMR)spectra(1H NMR and NOESY).And then,the effect of pH and two surfactants,including sodium dodecyl sulfate(SDS)and cetyltrimethylammonium bromide(CTAB)on the fluorescence intensity of AO/PP5A complex system were investigated.Finally,the complexation mode and energy of PP5A with AO and CTAB were calculated by molecular docking.The results showed that AO and PP5A formed a 1:1 host-guest complex.PP5A reduced or quenched the fluorescence intensity of AO in the pH range of 3~11.The addition of SDS and CTAB did not affect the fluorescence intensity of AO/PP5A complex significantly.The fluorescence changes generated by AO and PP5A complexation can provide a theoretical basis for the design of fluorescence sensors.

关 键 词：络合 分子对接 芳烃 吖啶橙 

分 类 号：O482.53[理学—固体物理]