五种植物粗多糖诱导小鼠骨髓来源树突状细胞成熟的体外筛选  

In vitro screening of mouse bone marrow-derived dendritic cells induced by five plant polysaccharides

作　　者：董晓筱 李博野 张季瑶 陈颖[3] 胡秦[1] 彭博 DONG Xiaoxiao;LI Boye;ZHANG Jiyao;CHEN Yin;HU Qin;PENG Bo(College of Life Sciences and Bioengineering,Beijing University of Technology,Beijing 100124,China;College of Environmental and Energy Engineering,Beijing University of Technology,Beijing 100124;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700)

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100124 [2]北京工业大学环境与能源工程学院,北京100124 [3]中国中医科学院中药研究所,北京100124

出　　处：《中国比较医学杂志》2020年第11期23-27,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(81760783);北京市自然科学基金(7182012);“重大新药创制”科技重大专项(2017ZX09101002-002-008)。

摘　　要：目的比较灵芝、枸杞、云芝、黄芪、香菇五种植物粗多糖对C57BL/6小鼠骨髓来源树突状细胞(bone marrow-derived dendritic cells,BMDC)的体外诱导功能。方法采用GM-CSF体外诱导和培养C57BL/6小鼠BMDC细胞,体外培养6 d后,分别加入灵芝、枸杞、云芝、黄芪和香菇五种植物粗多糖进行培养,24 h后,采用流式细胞术检测细胞表面CD80、CD86及MHCII分子的表达,采用ELISA检测BMDC细胞培养上清中细胞因子IL-6、IL-12和TFN-α的含量。结果云芝多糖可显著诱导BMDC细胞表明的CD80、CD86和MHCII分子表达上调,并刺激BMDC细胞释放IL-6,IL-12p40和TFN-α。枸杞多糖、灵芝多糖和香菇多糖仅显著性的诱导IL-6释放增加。结论云芝多糖对BMDC具有较强的诱导作用,具有潜在的疫苗佐剂活性。Objective To investigate the adjuvant activity of polysaccharides from Ganoderma lucidum,Lycium barbaru,Versicolor,Astragalus and Lentinan on bone marrow-derived dendritic cells(BMDCs)from C57 BL/6 mice.Methods Bone marrow cells from C57 BL/6 mice were cultured in the presence of granulocyte-macrophage colonystimulating factor GM-CSF.After 6 days,immature BMDCs were collected and co-cultured with the polysaccharides of G.lucidum,L.barbaru,Versicolor,Astragalus or Lentinula.Cp G oligodeoxynucleotides were used as a positive control.The cell surface expression of CD80,CD86 and MHCII were detected by fluorescence-activated cell sorting analysis.The release of cytokines interleukin(IL)-6,IL-12 and tumor necrosis factor TFN-αwere measured by enzyme-linked immunosorbent assay.Results Versicolor polysaccharides significantly upregulated the surface expression of CD80,CD86 and MHCII on BMDCs,as well as the release of the cytokines IL-6,IL-12 and TFN-αcompared with the control group.Conclusions Versicolor polysaccharides promoted the maturation of BMDCs in vitro and may have potential as an adjuvant for vaccine preparations.

关 键 词：多糖 树突状细胞 云芝多糖 佐剂 

分 类 号：R-33[医药卫生]