跑台运动对糖尿病大鼠学习记忆及海马炎症因子和NGF表达的影响  

Effects of treadmill exercise on learning,memory,inflammation,and NGF expression in the hippocampus of diabetic rats

作　　者：赵军 褚亚君 梁晋裕[1] 付芳 ZHAO Jun;CHU Yajun;LIANG Jinyu;FU Fang(Physical Education Department,Jinzhong College,Jinzhong 030619,China;Physical Education Institute,North University of China,Taiyuan 030051)

机构地区：[1]晋中学院体育系,山西晋中030619 [2]中北大学体育学院,太原030051

出　　处：《中国比较医学杂志》2020年第11期65-71,共7页Chinese Journal of Comparative Medicine

基　　金：山西省体育局课题(18TY131)。

摘　　要：目的探讨8周跑台运动对1型糖尿病模型大鼠学习记忆能力、海马炎症因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平及神经生长因子(NGF)表达的影响。方法44只成年雄性SD大鼠随机分成对照组、运动组、STZ组和STZ运动组,采用腹腔注射STZ诱导大鼠1型糖尿病模型,然后运动组及STZ运动组大鼠进行8周跑台运动。运动结束后采用Y迷宫实验评估大鼠学习记忆能力,ELISA法进行海马炎症因子IL-6及TNF-α水平的测定,免疫组化法检测海马NGF的表达。结果与对照组比较,Y迷宫实验中,STZ组大鼠达到学会标准训练次数及30次错误次数均显著增多(P<0.01),海马IL-6、TNF-α水平显著增加,海马CA1、CA3及DG区NGF个数及面积均显著下降(P<0.01);而8周跑台运动可改善STZ诱导的糖尿病大鼠学习记忆能力,海马TNF-α水平显著下降(P<0.05),而IL-6水平无显著改变(P>0.05),海马CA1、CA3及DG区NGF表达显著增强(P<0.05,P<0.01)。结论跑台运动可通过增强海马NGF表达及抗炎能力改善1型糖尿病大鼠的学习记忆能力。Objective To observe the effects of treadmill exercise on learning,memory,and expression levels of inflammatory cytokine interleukin 6(IL-6),tumor necrosis factor alpha(TNF-α),and nerve growth factor(NGF)in the hippocampus of type 1 diabetic rats.Methods Forty-four adult male Sprague–Dawley rats were randomly divided into control,exercise,STZ,and STZ exercise(STZ+E)groups(n=11).A rat model of type 1 diabetes was established by STZ intraperitoneal injection.Exercise and STZ+E groups were subjected to 8 weeks of treadmill exercise.Subsequently,learning and memory were evaluated by Y maze test,IL-6 and TNF-αlevels were measured by enzyme-linked immunosorbent assay,and NGF expression was detected by immunohistochemical staining.Results Compared with the control group,STZ group rats exhibited significantly increased standard training times and error times in 30 trials(P<0.01),IL-6 and TNF-αwere levels significantly increased and the number and area of NGF expression in hippocampal CA1,CA3,and dentate gyrus was decreased(P<0.01).Treatment of STZ-induced diabetic rats with exercise improved STZ-induced declines in learning and memory and significantly decreased TNF-αexpression(P<0.05).However,hippocampal IL-6 expression in STZ+E group rats was not significantly different(P>0.05),and NGF expression in the hippocampus CA1,CA3,and dentate gyrus was significantly upregulated(P<0.05).Conclusions Treadmill exercise can improve the learning and memory ability of type I diabetic rats by enhancing NGF expression and anti-inflammatory abilities in the hippocampus.

关 键 词：1型糖尿病大鼠 跑台运动 学习记忆 炎症因子 NGF表达 海马 

分 类 号：R-33[医药卫生]