美沙拉嗪栓剂与柳氮磺砒啶片剂对溃疡性结肠炎小鼠结肠组织中miR-155调控作用的研究  

Study on mesalazine suppository and sulfasalazine tablets on miR-15 regulation in colon tis⁃sue of ulcerative colitis mice

作　　者：赵丹[1] 郭洪梅[1] 夏辉[1] Zhao Dan;Guo Hongmei;Xia Hui(Xuzhou No.1 People’s Hospital,Xuzhou 221000,China)

机构地区：[1]江苏省徐州市第一人民医院,徐州221000

出　　处：《广西医科大学学报》2020年第11期1967-1972,共6页Journal of Guangxi Medical University

基　　金：江苏省徐州市科技项目(No.XZZDY1610)。

摘　　要：目的:探讨美沙拉嗪栓剂和柳氮磺砒啶片剂调控溃疡性结肠炎(UC)小鼠结肠组织中的miR-155表达及其治疗作用。方法:建立UC小鼠模型后,阴性对照组(N-Con组)和miR-155 inhinbition组小鼠分别经肠道灌入阴性对照病毒悬液和miR-155抑制剂基因的慢病毒悬液;美沙拉嗪组和柳氮磺砒啶组小鼠分别灌胃给药美沙拉嗪和柳氮磺砒啶片剂。治疗后,评估各组小鼠疾病活动度指数(DAI);分别测定各组小鼠结肠组织病理学、miR-155表达及白介素-6(IL-6)、白介素-21(IL-21)的含量。结果:与Con组比较,Mod组、N-Con、miR-155 inhibition、美沙拉嗪和柳氮磺吡啶组小鼠DAI均明显增加(P<0.05);UC模型小鼠经miR-155 inhinbition、柳氮磺吡啶和美沙拉嗪干预后DAI均明显降低(P<0.05)。相较于Con组,Mod组、N-Con组小鼠结肠组织中miR-155 mRNA水平、IL-6和IL-21含量均上调(P<0.01);相较于Mod组,miR-155 inhinbition组、柳氮磺砒啶组和美沙拉嗪组小鼠的miR-155 mRNA水平、IL-6和IL-21含量均下调(P<0.01),miR-155 inhinbition组变化更显著(P<0.05);美沙拉嗪组和柳氮磺砒啶组比较,差异无统计学意义(P>0.05)。结论:美沙拉嗪栓剂和柳氮磺砒啶片剂均可通过抑制UC小鼠结肠组织中的miR-155表达,有效治UC,且美沙拉嗪栓剂更显著,疗效更佳。Objective:To investigate the expression and therapeutic effect of miR-155 in colon tissues of ulcerative colitis(UC)mice treated with mesalazine suppository and sulfasalazine tablets.Methods:After the establishment of UC mice model,mice in negative control group(N-Con group)and miR-155 inhibition group were infused with negative control virus suspension and lentivirus suspension of miR-155 inhibitor genethrough intestines respectively.Mice in mesalazine suppository group and sulfasalazine tablets group were administered with mesalazine suppository and sulfasalazine tablets by gavage respectively.After treatment,disease activity index(DAI)was evaluated respectively in each group.Histopathological changes,miR-155 expression level as well as the contents ofinterleukin-6(IL-6)andinterleukin-21(IL-21)were determined respectively in mice.Results:Compared with Con group,DAI in Mod group,N-Con group,miR-155 inhibition group,mesalazine group and sulfasalazine group increased significantly(P<0.05).The DAI of UC model mice decreased significantly after intervention with miR-155 inhibition,sulfasalazine and mesalazine(P<0.05).Compared with Con group,the levels of miR-155,IL-6 and IL-21 in colonic tissue of mice in Mod group and N-Con group increased(P<0.01).Compared with Mod group,the levels of miR-155,IL-6 and IL-21 in miR-155 inhibition group,sulfasalazine group and mesalazine group decreased(P<0.01),and the changes in miR-155 inhibition group were more significant(P<0.05).There was no significant difference between mesalazine group and sulfasalazine group(P>0.05).Conclusion:Both mesalazine suppository and sulfasalazine tablets can effectively treat UC by inhibiting the expression of miR-155 in the colon of UC mice,and mesalazine suppositoryis more effective.

关 键 词：美沙拉嗪 柳氮磺砒啶 溃疡性结肠炎 MIR-155 

分 类 号：R574.62[医药卫生—消化系统]