一株灰肉红菇内生真菌的分离鉴定及其抑菌活性研究  

Isolation,identification and its antibacterial activity of an endophytic fungus of Russula griseocarnosa

作　　者：吴晓贤 刘远超[1,2] 王傲 卓丽君 胡惠萍 WU Xiaoxian;LIU Yuanchao;WANG Ao;ZHUO Lijun;HU Huiping(State Key Laboratory of Applied Microbiology Southern China/Guangdong Provincial Key Laboratory of Microbial Safety and Health,Guangdong Institute of Microbiology,Guangzhou 510070,Guangdong,China;School of Biolagy and Biological Engineering,South China University of Technology,Guangzhou 510006,Guangdong,China;Jiaoling Tiehan Big Health Industry Investment Co.Ltd.,Jiaoling 514100,Guangdong,China)

机构地区：[1]广东省微生物研究所华南应用微生物国家重点实验室/广东省微生物安全与健康重点实验室,广东广州510070 [2]华南理工大学生物科学与工程学院,广东广州510006 [3]蕉岭铁汉大健康产业投资有限公司,广东蕉岭514100

出　　处：《生物资源》2020年第5期585-592,共8页Biotic Resources

基　　金：广东省科技计划项目(2019B121202005);广东扬帆计划引进创新创业团队项目(2017YT05S115)。

摘　　要：灰肉红菇(Russula griseocarnosa)是岭南地区著名的野生食药用真菌。本研究通过组织分离的方法,从灰肉红菇子实体分离获得一株内生真菌,经形态学和分子生物学分析鉴定为爪哇虫草(Cordyceps javanica)。以牛津杯抑菌圈法及二倍稀释法测试了其菌丝体胞内和胞外多糖粗提物对金黄色葡萄球菌(Staphylococcus aureus)和铜绿假单胞菌(Pseudomonas aeruginosa)的抑菌活性。结果表明:水溶性胞内、胞外多糖对金黄色葡萄球菌及铜绿假单胞菌均具有抑菌活性,最低抑菌浓度(MIC)为37.5 mg/mL。Russula griseocarnosa is a well-known wild edible and medicinal fungus in Lingnan area.An endophytic fungus strain,which was identified as Cordyceps javanica by morphology and molecular biology,was isolated from the fruiting body of Russula griseocarnosa by tissue isolation method.We detected the antimicrobial activity of intracellular and extracellular polysaccharides extracted from the mycelia of the endophyte against Staphylococcus aureus and Pseudomonas aeruginosa respectively by the Oxford Cup method.Their minimum inhibitory concentration(MIC) was also tested by the double dilution method.The results showed that both the water-soluble intracellular and extracellular polysaccharides had antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa,with a minimum inhibitory concentration(MIC) of 37.5 mg/mL.

关 键 词：爪哇虫草 抑菌活性 多糖 灰肉红菇 

分 类 号：S646.9[农业科学—蔬菜学]