鸭短喙侏儒综合征精制蛋黄抗体的制备及应用效果评价  

Preparation and Application Effect Evaluation of a Purified Egg Yolk Antibody against Duck Short Beak and Dwarfism Syndrome

作　　者：李丽琴 程雪娇 王宇鹏 孙珊珊 石青青 宋秀梅 于海超 王建 LI Li-qin;CHENG Xue-jiao;WANG Yu-peng;SUN Shan-shan;SHI Qing-qing;SONG Xiu-mei;YU Hai-chao;WANG Jian(Tianjin Speerise Challenge Biotechnology Co.,Ltd.,Tianjin300380,China)

机构地区：[1]天津市中升挑战生物科技有限公司,天津300380

出　　处：《畜牧与饲料科学》2020年第6期100-105,共6页Animal Husbandry and Feed Science

基　　金：天津市科技计划项目“科技重大专项与工程计划”(17ZXGSNC00110)。

摘　　要：为了有效预防鸭短喙侏儒综合征,采用新型鸭细小病毒毒株(SD03株)制备了一种鸭短喙侏儒综合征精制蛋黄抗体。采用琼脂扩散试验和中和试验进行蛋黄抗体效价的测定,分析蛋黄抗体的消长规律,确定最佳免疫程序、收蛋时间和临床应用效价标准,并对制备的3批供试品进行临床应用效果评价。结果表明:该研究成功制备了一种鸭短喙侏儒综合征精制蛋黄抗体,该抗体琼扩效价和中和效价均在三免15 d达到高峰,分别为1∶64和1∶640,由此确定高免蛋收集时间为三免15 d;基础免疫完成后,最佳加强免疫时间为每隔30 d进行一次加强免疫,可使抗体效价一直维持在较高水平;确定了抗体琼扩效价≥1∶32或中和效价≥1∶320为临床应用效价标准。3批供试品临床应用试验结果表明,该鸭短喙侏儒综合征精制蛋黄抗体安全性好,对鸭短喙侏儒综合征有良好的预防和紧急预防效果。In order to prevent duck short beak and dwarfism syndrome effectively,a purified egg yolk antibody against novel duck parvovirus(strain SD03)was prepared.Agar diffusion test and neutralization test were used to determine the titer of egg yolk antibody.The growth and decline law of egg yolk antibody was analyzed to determine the optimal immune program,highly immunized egg collection time and clinical application titer standard.The clinical application effect of three batches of the test samples was evaluated.The results showed that a purified egg yolk antibody against duck short beak and dwarfism syndrome was successfully prepared.Both of the agar diffusion titer and neutralizing titer of the antibody reached the peak on the 15th day of the third immunization,which were 1∶64 and 1∶640,respectively.Therefore,the collection time of highly immunized eggs was determined to be 15 days after the third immunization.After the completion of the basic immunization,the best enhanced immunization time was once every 30 days.The results showed that the titer of the antibody could be maintained at a high level after one time booster immunization.The agar diffusion titer of the antibody≥1∶32 or neutralizing titer≥1∶320 were determined as the clinical application titer standard.The results of clinical application tests of three batches of samples showed that the purified egg yolk antibody against duck short beak and dwarfism syndrome was safe,and had good prevention and emergency prevention effect on duck short beak and dwarfism syndrome.

关 键 词：鸭短喙侏儒综合征 蛋黄抗体 效价测定 临床应用 

分 类 号：S852.52[农业科学—基础兽医学] S858.322.657[农业科学—兽医学]