AA肉仔鸡肌肉组织中miR-133在不同饲养方式下的表达研究  

Study on the Expression of miR-133 in AA Broiler Muscle Tissue under Different Feeding Methods

作　　者：吴秋雪 张伟伟[1,2] 常鑫 张磊 邵淑丽[1,2] WU Qiuxue;ZHANG Weiwei;CHANG Xin;ZHANG Lei;SHAO Shuli(College of Life Sciences,Agriculture and Forestry,Qiqihar,Heilongjiang 161006;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar,Heilongjiang 161006)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《中国家禽》2020年第11期22-26,共5页China Poultry

基　　金：国家青年科学基金项目(31801148);黑龙江省自然科学基金项目(C2016057);黑龙江省教育厅基本业务专项(135209260);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项)(YSTSXK201874)。

摘　　要：为了研究AA肉仔鸡肌肉组织中miR-133在不同饲养方式下的表达情况,试验采用生物信息学的方法分析了鸡肌肉组织中miR-133基因信息,比对了miR-133前体序列的同源性,通过stem-loop实时定量RT-PCR法分析gga-miR-133c-3p在不同饲养方式的AA肉仔鸡肌肉组织中的表达水平。试验结果表明,共有4个miR-133前体序列,分别位于鸡基因组23号、2号.3号和20号染色体上,4个miR-133的同源性可达96.59%;散养方式的AA肉仔鸡的翅肌、腿肌中gga-miR-133c-3p表达显著低于笼养方式,胸肌、心肌中gga-miR-133c-3p的表达显著高于笼养方式。In order to study the expression of miR-133 in muscle tissue of AA broilers under different feeding methods, the bioinformatics method was used to analyze the miR-133 gene information in chicken muscle tissues, and the homology of miR-133 precursor sequences was compared. The expression level of gga-miR-133c-3p in muscle tissues of AA broilers with different feeding methods was analyzed by real-time quantitative RT-PCR with stem-loop.The study results showed that there were four miR-133 precursor sequences, which were located on chromosomes 23, 2, 3, and 20 of the chicken genome. The homology of the four miR-133 could reach 96.59%. The expression of gga-miR-133c-3p in the wing muscles and leg muscles of AA broilers was significantly higher than that in cage culture. The expression of gga-miR-133c-3p in breast muscle and myocardium was significantly higher than that in cage culture.

关 键 词：miR-133 饲养方式 肌肉组织 骨骼肌 肉仔鸡 

分 类 号：S831.2[农业科学—畜牧学]