microRNA455在黑色素瘤组织和细胞系中的表达及临床意义  

Expression and significance of microRNA455 in melanoma tissue and cell line

作　　者：单秀娟[1] 于亮[2] 孙怡[1] 王振华[1] 严庆涛[3] 王辉[1] SHAN Xiujuan;YU Liang;SUN Yi;WANG Zhenhua;YAN Qingtao;WANG Hui(Departments of Dermatology,Weifang People's Hospital,Weifang 261041,China;Clinical Laboratory,Weifang People's Hospital;Departments of Pediatric Surgery,Weifang People's Hospital)

机构地区：[1]潍坊市人民医院皮肤科,山东潍坊261041 [2]潍坊市人民医院检验科 [3]潍坊市人民医院小儿外科

出　　处：《潍坊医学院学报》2020年第5期349-351,共3页Acta Academiae Medicinae Weifang

基　　金：潍坊市卫生计生委科研项目计划课题(项目编号:2017wsjs125);潍坊市卫生计生委科研项目计划课题(项目编号:2016wsjs024);潍坊市科技发展计划项目课题(项目编号:2019YX007)。

摘　　要：目的探讨microRNA455(miR-455)在黑色素瘤中的表达及临床意义。方法通过实时荧光定量聚合酶链反应(qRT-PCR)法测定黑色素瘤组织和细胞系中miR-455的表达水平。结果与匹配的正常组织或正常表皮黑素细胞相比,黑色素瘤中miR-455显著下调,差异有显著性(P<0.05);另外,黑色素瘤细胞中miR-455表达增加,减少了细胞的增殖和侵袭(P<0.05)。结论在黑色素瘤中miR-455表达下调,并抑制黑色素瘤细胞的增殖和侵袭,miR-455的恢复可作为黑色素瘤患者的治疗方法。Objective To investigate the expression and significance of microRNA455(miR-455) in melanoma tissue and cell line.Methods In this study,the expression levels of miR-455 in melanoma tissues and cell lines were determined by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).Results Compared with matched normal tissues or normal epidermal melanocytes,miR-455 was significantly down-regulated in melanoma with a significant difference(P<0.05);in addition,increased expression of miR-455 in melanoma cells reduced cell proliferation and invasion(P<0.05).Conclusion These findings suggest that miR-455 is down-regulated in melanoma and inhibits the proliferation and invasion of melanoma cells.miR-455 recovery as a treatment for melanoma patients may be worth studying.

关 键 词：黑素细胞瘤 microRNA455 病理学 

分 类 号：R739.5[医药卫生—肿瘤]