响应面法优化[Cn mim]Cl(n=2,4,6)-K2 HPO4双水相法萃取无花果蛋白酶  

Optimization of[Cn min]Cl(n=2,4,6)-K2 HPO4 ionic liquid aqueous two-phase system extraction of ficin using response surface methodology

作　　者：曾杰 房海灵[1] 梁呈元[1] 亓希武[1] 郭强 于盱[1] Zeng Jie;Fang Hailing;Liang Chengyuan;Qi Xiwu;Guo Qiang;Yu Xu(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China;Junrong Huershan Fig Cooperatives,Zhenjiang 212013,China)

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014 [2]句容市虎耳山无花果专业合作社,江苏镇江212013

出　　处：《江苏林业科技》2020年第5期5-10,共6页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省农业科技自主创新资金项目“无花果高效栽培及副产物综合利用关键技术研究”[CX(18)3030];江苏省林业科技创新与推广项目“无花果良种筛选及深加工技术研究”(LYKJ[2018]35)。

摘　　要：以[C n mim]Cl(n=2,4,6)-K 2 HPO 4离子液体双水相法萃取无花果蛋白酶,以酶活回收率和纯化因子为评价指标,分别考察K 2 HPO 4质量浓度、离子液体种类及其质量浓度、酶添加量和pH等因素不同水平对萃取效果的影响,筛选中心实验值,通过Box-Behnken模块建立因素与响应值之间函数关系来优化萃取参数。结果显示,优化后最佳萃取条件如下:[C 4 mim]Cl为0.35 g/mL,K 2 HPO 4质量浓度为0.3 g/mL,酶添加量为2.5 mg/mL,pH为8.5,此条件下无花果蛋白酶酶活回收率为92.64%,纯化倍数为1.81。此研究结果为利用新型绿色溶剂萃取无花果蛋白酶提供理论指导。[C n mim]Cl(n=2,4,6)-K 2 HPO 4 ionic liquid aqueous two-phase method was used to extract ficin.The extraction effects of different K 2 HPO 4 concentration,ionic liquid type and concentration,enzyme addition and pH were evaluated by ficin activity recovery and purification factors,and the central experimental values were screened.The functional relationship between factors and response values was established by Box-Behnken to optimize the extraction parameters.Results showed that optimal extraction conditions were 0.3 g/mL K 2 HPO 4,0.35 g/mL ionic liquid[C 4 mim]Cl,2.5 mg/mL enzyme addition,pH 8.5,and under these conditions ficin activity recovery rate reached 92.64%,the purification factor reached 1.81.The research results could provide theoretical guidance for the extraction of ficin with novel green solvent.

关 键 词：离子液体双水相法 萃取 无花果蛋白酶 响应面 

分 类 号：O652.62[理学—分析化学] Q556.9[理学—化学]