miR-423-3p在乙型肝炎病毒相关肝癌肝移植组织中表达上调对患者预后的影响  

The up-regulation of miR-423-3p in hepatitis B virus-related liver cancer liver transplantation and its effect on the prognosis of patients

作　　者：柯瑞盛 陈重发 陈永泰 张思宇[1] 刘昭晖[1] 蔡秋程 江艺 吕立志 Ke Ruisheng;Chen Chongfa;Chen Yongtai;Zhang Siyu;Liu Zhaohui;Cai Qiucheng;Jiang Yi;Lv Lizhi(Department of General surgery,the First Affiliated Hospital of Xiamen University,Xiamen 361001,Fujian,China;Graduate School of Fujian Medical University,Fuzhou 350122,Fujian,China;Xiamen University School of Medicine,Xiamen 361001,Fujian,China;Department of Hepatobiliary Surgery,900 Hospital of the Joint Logistics Team,Fuzhou 350025,Fujian,China)

机构地区：[1]厦门大学附属第一医院普外科,福建厦门361001 [2]福建医科大学研究生院,福建福州350122 [3]厦门大学医学院,福建厦门361001 [4]中国人民解放军联勤保障部队第九〇〇医院(原南京军区福州总医院)肝胆外科,福建福州350025

出　　处：《实用器官移植电子杂志》2020年第6期466-471,共6页Practical Journal of Organ Transplantation(Electronic Version)

基　　金：福建省科技厅引导性项目(2019Y0068,2015Y0026);解放军后勤医学研究重点项目基金资助(CNJ15J002、14ZX22);福建医科大学科研启航基金项目(2017XQ2048)。

摘　　要：目的检测miR-423-3p在肝癌样本组织中的表达情况,探讨其可否作为乙型肝炎病毒(hepatitis B virus,HBV)肝细胞癌(hepatocellular carcinoma,HCC)肝移植患者诊疗的潜在分子靶标。方法StarBase v3.0数据库分析miR-423在肝癌样本组织中表达及预后价值,采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)实验方法检测106例肝癌肝移植组织和30例正常癌旁肝组织中miR-423-3p表达,结合患者的临床资料分析miR-423-3p表达与肝癌行肝移植患者病理特征和预后生存的关系。结果与邻近正常组织相比,qRT-PCR实验检测发现在肝癌组织中miR-423-3p表达增高。临床病理学特征分析结果表明miR-423-3p表达与无肿瘤包膜、较大肿瘤直径、较低分化程度和较高TNM分期密切相关(P<0.05)。进一步COX多因素回顾分析发现miR-423-3p表达,分化程度,TNM分期等是患者预后不良的独立预后危险因素(P<0.05)。采用Kaplan-Meier方法和秩和检验进行统计分析,miR-423-3p过表达肝癌肝移植患者累积生存率显著低于低表达患者(P<0.01)。结论miR-423-3p可能参与肝癌的发生和发展过程,可以作为肝癌肝移植患者疾病预后标志物和临床治疗靶标。Objective To detect the expression of miR-423-3p in hepatocellular carcinoma tissues and cells,to explore its role in the occurrence and development of liver cancer,and to explore whether it can be used as a potential molecular target for the detection and treatment of liver cancer in liver transplantation.Methods The StarBase V 3.0 database was used to analyze the expression and prognostic value of miR-423 in liver cancer sample tissues,and quantitative real-time polymerase chain reaction(qRT-PCR)experimental method was applied to detect the expression of miR-423-3p in 106 cases of liver cancer liver transplantation tissues and in 30 normal adjacent liver tissues.The relationship between the expression of miR-423-3p and the pathological characteristics and prognosis of liver cancer patients undergoing liver transplantation was analyzed based on the clinical data of the patients.Results Compared with adjacent non-tumor tissues,qRT-PCR detected that miR-423-3p expression was increased in liver cancer tissues.The analysis of clinicopathological characteristics of HCC patients showed that miR-423-3p expression was closely related to tumor-free envelope,larger tumor diameter,lower differentiation and higher TNM stage(P<0.05).Further COX multivariate retrospective analysis revealed that miR-423-3p expression,degree of differentiation and TNM stage were independent prognostic risk factors for patients with poor prognosis(P<0.05).Using Kaplan-Meier analysis,the cumulative survival rate of miR-423-3p overexpressing liver cancer patients was significantly lower than that of patients with low expression(P<0.01).Conclusion Ove rexpression of miR-423-3p may be involved in the occurrence and development of HBV-related HCC,and it can be used as a prognostic marker and clinical target for liver cancer patients with liver transplantation.

关 键 词：肝细胞癌 肝移植 miR-423-3p 预后 生物信息学分析 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]