红系祖细胞诱导非小细胞肺癌进展作用机制探讨  

Role and mechanism of erythroid progenitor cells in inducing progression of non-small cell lung cancer

作　　者：金刚 脱广鑫 朱自江[1] JIN Gang;TUO Guang-xin;ZHU Zi-jiang(Department of Chest SurgeryⅡ,Gansu Provincial Hospital,Lanzhou730000,P.R,China;College of Clinical Medicine,Gansu University of Chinese Medicine,Lanzhou730000,P.R,China)

机构地区：[1]甘肃省人民医院胸外二科,甘肃兰州730000 [2]甘肃中医药大学临床医学院,甘肃兰州730000

出　　处：《中华肿瘤防治杂志》2020年第20期1629-1634,共6页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的免疫治疗只对部分人群有效,寻找新的免疫治疗靶点成为研究重点。本研究探讨红系祖细胞诱导非小细胞肺癌(non small-cell lung cancer,NSCLC)进展的作用及机制。方法选取2015-03-01-2018-03-01来甘肃省人民医院就诊的NSCLC初治患者做为研究对象;流式细胞术检测CD45^+红系祖细胞(CD45^+CD71+TER119+细胞)、CD45^-红系祖细胞(CD45^-CD71+TER119+细胞)、T细胞和B细胞的比例;免疫组化检测Ki-67比例;MTT实验检测增殖水平。结果流式细胞术结果显示,NSCLC不合并贫血组红系祖细胞、CD45^+和CD45^-红系祖细胞比例分别为0.526%、0.428%和0.099%,NSCLC合并贫血组比例分别为1.770%、1.577%和0.206%,差异均有统计学意义,χ^2值分别为6.818、6.593和0.030,P值分别为0.009、0.010和4.711。NSCLC合并贫血患者肿瘤组织Ki-67阳性的比例为11.765%,NSCLC合并不贫血组的比例为20.689%,差异有统计学意义,χ^2=5.796,P=0.016。相关性分析结果显示,红系祖细胞比例与Ki-67阳性比例呈正相关,r=0.747;P=0.013;MTT结果显示,对照组NSCLC细胞增殖率为20.037%,CD45^-EPC组增殖率为40.671%,差异有统计学意义,χ^2=9.524,P=0.002;Artemin阻断组增殖率为21.175%,相对于CD45^-EPC组差异有统计学意义,χ^2=8.515,P=0.004。流式细胞术结果显示,CD45^+红系祖细胞能过通过分泌ROS,抑制T细胞的增殖,CD45^-红系祖细胞对T细胞增殖没有明显的作用;CD45^+红系祖细胞和CD45^-红系祖细胞对B细胞的作用均差异无统计学意义。结论红系祖细胞在NSCLC合并贫血患者外周血中比例显著升高,CD45^+和CD45^-红系祖细胞分别通过抑制T细胞免疫和分泌Artemin,促进NSCLC的进展。OBJECTIVE Immunotherapy is only effective for some people,and the search for new immunotherapy targets has become the focus of research.The purpose of this study was to investigate the role and mechanism of erythroid progenitor cells in inducing the progression of non-small cell lung cancer(NSCLC).METHODS Patients with NSCLC from March 2015 to March 2018 were selected as the study subjects.Flow cytometry was used to detect the proportion of CD45^+ erythroid progenitor cells(CD45^+CD71^+TER119^+cells),CD45-erythroid progenitor cells(CD45^-CD71^+TER119^+cells),T cells and B cells.Ki-67 ratio was detected by immunohistochemistry and proliferation level was detected by MTT assay.RESULTS The results of flow cytometry showed that the percentage of erythroid progenitor cells,CD45^+ and CD45^- erythroid progenitor cells in NSCLC without anemia group was 0.526%,0.428% and 0.099%,and that in NSCLC with anemia group was 1.770%,1.577% and 0.206%,with significant statistical differences (χ^2=6.818,6.593 and 0.030;Pvalues were 0.009,0.010 and 4.711).The positive rate of Ki-67 in tumor tissue of patients with NSCLC complicated with anemia was 11.765%,and that of non-anemia group was 20.689%,the difference was statistically significant(χ^2=5.796,P=0.016).The results of correlation analysis showed that the proportion of erythroid progenitor cells was positively correlated with that of Ki-67 positive cells(r=0.747,P=0.013).The results of MTT showed that the proliferation rate of NSCLC cells in the control group was 20.037%,and that in the CD45-EPC group was 40.671%.Compared with the control group,the difference was statistically significant(χ^2=9.524,P=0.002).The retention rate of the Artein blocking group was 21.175%,and the difference was statistically significant with respect to the CD45-EPC group(χ^2=8.515,P=0.004).The results of flow cytometry showed that CD45^+ erythroid progenitor cells could inhibit the proliferation of T cells by secreting ROS,while CD45-erythroid progenitor cells had no obvious effect on the prolifer

关 键 词：红系祖细胞 非小细胞肺癌 贫血 免疫 T细胞 

分 类 号：R734.2[医药卫生—肿瘤]