粪便嗜酸性粒细胞与婴幼儿牛奶蛋白过敏性肠炎的相关性  

Correlation between eosinophil of stool and infant milk protein allergic enteritis

作　　者：曹彤[1] 林谦 朱航 潘键 刘志峰[2] 金玉[2] Cao Tong;Lin Qian;Zhu Hang;Pan Jian;Liu Zhifeng;Jin Yu(Department of Clinical Laboratory,Children′s Hospital of Nanjing Medical University,Nanjing 210008,China;Department of Gastroenterology,Children′s Hospital of Nanjing Medical University,Nanjing 210008,China)

机构地区：[1]南京医科大学附属儿童医院检验科,210008 [2]南京医科大学附属儿童医院消化科,210008

出　　处：《中华实用儿科临床杂志》2020年第22期1718-1721,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的探讨牛奶蛋白过敏性肠炎患儿粪便中嗜酸性粒细胞(EOS)的数量变化,为牛奶蛋白过敏性肠炎的诊断和治疗提供依据。方法选取2018年7月至2019年6月南京医科大学附属儿童医院收治的70例牛奶蛋白过敏性肠炎患儿(过敏组)、同期就诊的50例非过敏普通腹泻患儿(非过敏组)及50例健康体检儿童(健康对照组),采用全自动血细胞分析仪计数3组儿童外周血白细胞(WBC)、血红蛋白(Hb)、血小板(PLT)、EOS及EOS%;Gomori染色法计数3组儿童及过敏组治疗后粪便中的EOS,分析粪便中EOS与牛奶蛋白过敏性肠炎的相关性。结果过敏组外周血EOS计数(0.71±0.74)×10^9/L、EOS%(7.56±5.96)%、PLT(382.96±85.98)×10^9/L;非过敏组外周血EOS计数(0.31±0.18)×10^9/L、EOS%(3.53±2.26)%、PLT(315.12±69.81)×10^9/L;健康对照组外周血EOS计数(0.31±0.15)×10^9/L、EOS%(3.66±1.65)%、PLT(307.56±85.20)×10^9/L。过敏组外周血EOS、EOS%、PLT均高于非过敏组和健康对照组(F=13.606、19.055、16.074,均P<0.05)。过敏组粪便EOS计数[(10.75±17.17)个/50个1000倍视野]明显高于非过敏组[(1.16±3.09)个/50个1000倍视野],差异有统计学意义(F=17.25,P<0.05),而健康对照组患儿粪便未见EOS。过敏组患儿回避牛奶4周后,粪便中EOS计数明显下降[(2.26±5.32)个/50个1000倍视野],与治疗前比较差异有统计学意义(t=5.822,P<0.05);而血EOS计数[(0.56±0.47)×10^9/L]下降不明显,差异无统计学意义(t=1.981,P>0.05)。结论牛奶蛋白过敏性肠炎患儿粪便中EOS数量明显升高,经治疗后下降,有助于指导过敏性肠炎临床诊断及治疗效果的评估。Objective To investigate the changes of eosinophil(EOS)in feces of children with allergic enteritis caused by milk protein,and to provide basis for diagnosis and treatment of allergic enteritis.Methods From July 2018 to June 2019,70 children with milk protein allergic enteritis(allergic group),50 children with non allergic common diarrhea(non-allergic group)and 50 children with healthy physical examination(healthy control group)were selected from Children′s Hospital of Nanjing Medical University.White blood cell(WBC),hemoglobin(Hb),blood platelet(PLT),EOS and the percentage of EOS(EOS%)of the 3 groups were counted by automatic hematology analyzer.The EOS in the feces of the 3 groups and the allergic group after treatment were counted by Gomori staining,and the correlation between EOS in the feces and milk protein allergic enteritis was analyzed.Results For allergic group,EOS count in peripheral blood was(0.71±0.74)×10^9/L,EOS%was(7.56±5.96)%,and PLT was(382.96±85.98)×10^9/L.For non-allergic group,EOS was(0.31±0.18)×10^9/L,EOS%was(3.53±2.26)%,PLT was(315.12±69.81)×10^9/L.For healthy control group,EOS was(0.31±0.15)×10^9/L,EOS%was(3.66±1.65)%,and the PLT was(307.56±85.20)×10^9/L.The number of EOS,EOS%and PLT in allergic group were higher than those in non-allergic group and healthy control group(F=13.606,19.055,16.074,all P<0.05).The number of EOS in feces of allergic group[(10.75±17.17)/50 fields at×1000]was significantly higher than that of non-allergic group[(1.16±3.09)/50 fields at×1000],the difference was statistically significant(F=17.25,P<0.05),and there was no EOS in feces of healthy control group.After 4 weeks of avoidance of milk,EOS in feces decreased significantly in allergic group[(2.26±5.32)/50 fields at×1000],the difference was statistically significant(t=5.822,P<0.05),while EOS in blood decreased[(0.56±0.47)×10^9/L],the difference was not statistically significant(t=1.981,P>0.05).Conclusions EOS in fecal of children with milk protein allergic enteritis increased obviously an

关 键 词：嗜酸性粒细胞 婴幼儿 牛奶蛋白过敏 过敏性肠炎 

分 类 号：R725.7[医药卫生—儿科]