Identification of Hub Genes and Key Pathways Associated with Peripheral T-cell Lymphoma  

作　　者：Hai-xia GAO Meng-bo WANG Si-jing LI Jing NIU Jing XUE Jun LI Xin-xia LI 

机构地区：[1]Department of Pathology,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China [2]Xinjiang Medical University,Urumqi 830011,China [3]Deparment of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases,The First Affiliated Hospital,Shihezi University School of Medicine,Shihezi 832002,China [4]Department of Ultrasound,The First Affiliated Hospital,Shihezi University School of Medicine,Shihezi 832002,China

出　　处：《Current Medical Science》2020年第5期885-899,共15页当代医学科学（英文）

基　　金：The study was supported by grants from the National Natural Science Foundation of China(No.81660036);the Project of the Bingtuan Science and Technology(No.2019DB012).

摘　　要：Peripheral T-cell lymphoma(PTCL)is a very aggressive and heterogeneous hematological malignancy and has no effective targeted therapy.The molecular pathogenesis of PTCL remains unknown.In this study,we chose the gene expression profile of GSE6338 from the Gene Expression Omnibus(GEO)database to identify hub genes and key pathways and explore possible molecular pathogenesis of PTCL by bioinformatic analysis.Diferentially expressed gencs(DEGs)between PTCL and normal T cells were selected using GEO2R tool.Gene ontology(GO)analysis and Kyoto Encyclopedia of Gene and Genome(KEGG)pathway analysis were performed using Database for Annotation,Visualization and Integrated Discovery(DAVID).Moreover,the Search Tool for the Retrieval of Interacting Genes(STRING)and Molecular Complex Detection(MCODE)were utilized to construct protein-protein interaction(PPI)network and perform module analysis of these DEGs.A total of 518 DEGs were identifed,including 413 down-regulated and 105 up-regulated gencs.The down-regulated genes were enriched in osteoclast differentiation,Chagas disease and mitogen-activated protein kinase(MAPK)signaling pathway.The up-regulated genes were mainly associated with extracellular matrix(ECM)-receptor interaction,focal adhesion and pertussis.F our important modules were detected from the PPI network by using MCODE software.Fifteen hub genes with a high degree of connectivity were selected.Our study identifed DEGs,hub genes and pathways associated with PTCL by bioinformatic analysis.Results provide a basis for further study on the pathogenesis of PTCL.

关 键 词：peripheral Tcell lymphomas bioinformatic analysis protein-protein interaction hub genes pathwayg 

分 类 号：R73[医药卫生—肿瘤]