紫色红曲霉的微波诱变及其产红曲色素发酵条件的优化  

Microwave mutagenesis of Monascus purpureus and optimization of fermentation conditions for Monascus pigment production

作　　者：武金霞[1] 王琰 谭政浩 刘爽[1] 隋思强 张贺迎[2] WU Jinxia;WANG Yan;TAN Zhenghao;LIU Shuang;SUI Siqiang;ZHANG Heying(School of Life Sciences,Hebei University,Baoding 071002,China;Research Center for Biotechnology,Hebei University,Baoding 071002,China)

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北大学生物技术研究中心,河北保定071002

出　　处：《河北大学学报（自然科学版）》2020年第6期618-624,共7页Journal of Hebei University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31573227)。

摘　　要：以紫色红曲霉S1为出发菌株进行微波诱变,筛选出产红曲色素能力最高的突变株紫色红曲霉S1-29,其红曲色素值达3240U/g,比出发菌株提高了57%.针对菌株S1-29固态发酵培养产红曲色素的条件进行优化,添加不同的碳源、氮源以及无机盐,研究添加物对产红曲色素能力的影响,并进行正交实验,得到最佳发酵条件:在大米基质中添加葡萄糖、蛋白胨、硫酸镁和磷酸氢二钾,使其质量分数分别为4%,6%,0.4%和0.3%,32℃培养11d,菌株S1-29红曲色素值达到4003U/g,比优化前提高23.55%.The microwave mutagenesis was carried out when Monascus purpureus S1 was used as the original strain.M.purpureus S1-29 was screened with the highest Monascus pigment value of 3240 U/g dry base,which was increased by 57%compared with that of the original strain.The conditions for the production of Monascus pigment by solid-state fermentation of strain S1-29 were optimized.The effects of carbon sources,nitrogen sources and inorganic salts on pigment production were investigated and orthogonal experiments were carried out.The results showed that when 4%glucose,6%peptone,0.4%magnesium sulfate and 0.3%dipotassium hydrogen phosphate were added into the rice media and cultured at 32℃for 11 days,the Monascus pigment value of M.purpureus S1-29 reached 4003 U/g dry base.which was increased by 23.55%compared with that of control.

关 键 词：紫色红曲霉 红曲色素 发酵优化 

分 类 号：Q939.97[生物学—微生物学]