Integrated transcriptome, small RNA, and degradome analysis to elucidate the regulation of rice seedling mesocotyl development during the passage from darkness to light  

作　　者：Yusong Lyu Xiangjin Wei Min Zhong Shipeng Niu Shakeel Ahmad Gaoneng Shao Guiai Jiao Zhonghua Sheng Lihong Xie Shikai Hu Yawen Wu Shaoqing Tang Peisong Hu 

机构地区：[1]State Key Laboratory of Rice Biology,China National Center for Rice Improvement,China National Rice Research Institute,Hangzhou 310006,Zhejiang,China [2]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Hubei,China

出　　处：《The Crop Journal》2020年第6期918-928,共11页作物学报（英文版）

基　　金：financially supported by the National S&T Major Project of China(2016ZX08001006);the National Key Research and Development Program of China(2016YFD0101801 and 2017YFD0100300);the Agricultural Science and Technology Innovation Program of CAAS。

摘　　要：The mesocotyl,a structure located between the basal part of the seminal root and the coleoptile node of seedlings,contributes to pushing the shoot tip through the soil surface,a function that is essential for the uniform emergence of direct-seeded rice.Its elongation is inhibited by light and induced in darkness.This investigation of an indica rice(P25)with vigorous mesocotyl elongation was aimed at identifying the"omics"basis of its lightinduced growth inhibition.A transcriptomic comparison between mesocotyl tissues that had developed in the dark and then been exposed to light identified many differentially expressed genes(DEGs)and differentially abundant micro RNAs(mi RNAs).Degradome sequencing analysis revealed 27 negative mi RNA-target pairs.A co-expression regulatory network was constructed based on the mi RNAs,their corresponding targets,and DEGs with a common Gene Ontology term.It suggested that auxin and light,probably antagonistically,affect mesocotyl elongation by regulating polyamine oxidase activity.

关 键 词：Oryza sativa L. MESOCOTYL TRANSCRIPTOME MicroRNAome DEGRADOME 

分 类 号：S511[农业科学—作物学]