LncRNA-UCA1、HMGB1/RAGE在非小细胞肺癌中的表达和意义  

Expression and significance of lncRNA-UCA1,HMGB1/RAGE in non-small cell lung cancer

作　　者：牟沧浪 杜春华[1] 高苏苏 孙威 于洁[1] 张亚楠[1] 于文成[1] Mou Canglang;Du Chunhua;Gao Susu;Sun Wei;Yu Jie;Zhang Yanan;Yu Wencheng(Department of Respiratory and Critical Care Medicine,the Affiliated Hospital of Qingdao University)

机构地区：[1]青岛大学附属医院呼吸与危重症医学科,青岛266000

出　　处：《重庆医科大学学报》2020年第11期1545-1550,共6页Journal of Chongqing Medical University

基　　金：青岛市市南区科技计划资助项目(编号:2016-3-047-YY)。

摘　　要：目的:明确长链非编码RNA-尿路上皮癌胚抗原1(long non-coding RNA-human urothelial carcinoma associated 1,LncRNA-UCA)、高迁移率族蛋白B1(high mobility group box-B1,HMGB1)、晚期糖基化终末产物受体(receptor for advanced glycation end-products,RAGE)、核因子-κB(nuclear factor kappa-B,NF-κB)p65在人体非小细胞肺癌(non-small cell lung cancer,NSCLC)组织及癌旁正常组织中的表达,探究其与NSCLC临床特点、病理类型等的关系。方法:选取2018年5月至2018年8月青岛大学附属医院收治的40例NSCLC患者,采集肺癌组织及癌旁正常组织标本各40例。应用免疫组化法检测10例患者肺癌组织及正常组织中的HMGB1、RAGE定位及表达量。应用Western blot检测12例NSCLC患者的肺癌组织及正常组织中HMGB1、RAGE、NF-κB p65蛋白的表达情况。采用qRT-PCR测定40例NSCLC患者肺癌组织及正常组织中lncRNA-UCA1、HMGB1、RAGE、NF-κB p65的mRNA表达量。结果:①免疫组化及Western blot检测显示,HMGB1(1459.72±366.67)、NF-κB p65(479.52±265.07)在NSCLC中的表达量较正常组织(847.60±313.66、135.53±109.61)明显升高(t=5.470,P=0.000;t=4.063,P=0.002),RAGE(127.49±43.91)在NSCLC中的表达较正常组织(257.61±64.84)明显降低(t=-11.461,P=0.000)。②qRTPCR检测显示,LncRNA-UCA1(4.85±2.31)、HMGB1(4.64±2.10)、NF-κB p65(4.22±2.10)在NSCLC中的表达较正常组织(1.00±0.00、1.00±0.00、1.00±0.00)明显升高(t=10.521,P=0.000;t=10.949,P=0.000;t=8.273,P=0.000),RAGE(0.41±0.19)在NSCLC中的表达较正常组织(1.00±0.00)降低(t=-19.754,P=0.000)。③LncRNA-UCA1、HMGB1、NF-κB p65的表达量在腺癌组织(2.93±1.40、3.06±1.36、2.17±0.87)高于鳞癌组织(6.77±2.22、6.16±2.20、5.96±2.90)(t=3.722,P=0.001;t=3.114,P=0.004;t=3.348,P=0.002),大于3 cm肿瘤组织(6.70±2.14、6.28±1.80、6.07±2.17)高于不足3 cm肿瘤组织(3.11±1.40、3.21±1.43、2.28±0.86)(t=-5.367,P=0.000;t=-5.181,P=0.000;t=-5.623,P=0.000),有淋巴结转移癌组织(6.38±2.25、6.04Objective:To clarify the expression of long non-coding RNA-Human urothelial carcinoma associated 1(LncRNA-UCA),high mobility group box-B1(HMGB1),receptor for advanced glycation end-products(RAGE)and nuclear factor kappa-B(NF-κB)p65 in human non-small cell lung cancer(NSCLC)tissues and normal tissues,and to explore its relationship with the clinical features and pathological types of NSCLC.Methods:Forty patients with NSCLC admitted to our hospital from May 2018 to August 2018 were enrolled.Cancer tissues and adjacent normal tissues of 40 patients were respectively collected.The expression of HMGB1 and RAGE in cancer tissues and normal tissues 10 patients were detected by immunohistochemistry.The expression of HMGB1,RAGE,NF-κB p65 protein in cancer tissues and normal tissues of 12 patients was detected by Western blot.The mRNA expression of LncRNA-UCA1,HMGB1,RAGE and NF-κB p65 in cancer tissues and normal tissues of 40 patients were deter mined by quantitative real-time polymerase chain reaction(qRT-PCR).Results:Immunohistochemistry and Western blot analysis showed that expression of HMGB1(1459.72±366.67)and NF-κB p65(479.52±265.07)in the NSCLC tissues was significantly higher than that in the adjacent normal tissues(847.60±313.66,135.53±109.61)(t=5.470,P=0.000;t=4.063,P=0.002),while expression of RAGE(127.49±43.91)in the NSCLC tissues was significantly lower than that in the adjacent normal tissues(257.61±64.84)(t=-11.461,P=0.000).The qRT-PCR detection showed that expression of LncRNA-UCA1(4.85±2.31),HMGB1(4.64±2.10)and NF-κB p65(4.22±2.10)in the NSCLC tissues was significantly higher than that in the adjacent normal tissues(1.00±0.00),while expression of RAGE(0.41±0.19)in the NSCLC tissues was significantly lower than that in the adjacent normal tissues(1.00±0.00)(t=-19.754,P=0.000).The expression of LncRNA-UCA1,HMGB1 and NF-κB p65 in the adenocarcinoma(2.93±1.40,3.06±1.36,2.17±0.87)was higher than that in the squamous cell carcinoma(6.77±2.22,6.16±2.20,5.96±2.90)(t=3.722,P=0.001;t=3.114,P

关 键 词：肺癌 长链非编码RNA-尿路上皮癌胚抗原1 高迁移率族蛋白B1 晚期糖基化终末产物受体 NF-ΚB信号通路 

分 类 号：R734.2[医药卫生—肿瘤]