精酿啤酒糟和蓝莓果渣混合发酵生产饲料蛋白的研究  

Study on Mixed Fermentation of Craft Beer's Grains and Blueberry Residue to Produce Feed Protein

作　　者：宋宇[1] 曲贵伟[1] SONG Yu;QU Gui-wei(Urban Construction College,Eastern Liaoning University,Dandong 118003,Liaoning,China)

机构地区：[1]辽东学院城市建设学院,辽宁丹东118003

出　　处：《食品研究与开发》2020年第23期161-165,共5页Food Research and Development

摘　　要：为合理利用精酿啤酒糟和蓝莓果渣的生产废弃物,采用普通高温放线菌、白地霉和植物乳杆菌的混合菌种经过两次不同发酵生产饲料蛋白,探讨发酵时间、菌种比、精酿啤酒糟和蓝莓渣的比值及混合量、麸皮量和菌种接种量对产物真蛋白含量的影响,通过Plackett-Burman和Box-Behnken试验设计及响应面分析,结果确定出最优发酵工艺条件为:精酿啤酒糟和蓝莓渣的质量比为1.42∶1,精酿啤酒糟和蓝莓渣的混合量是87.66%,麸皮量为9.9%,白地霉和植物乳杆菌的质量比为4∶1,最佳接种量为5%时,真蛋白含量可达40.15%;并得到产物真蛋白含量与各因素之间的二次多项式模型,通过试验结果对模型进行验证,实际值与理论值非常接近,模型可靠性高,可较好预测发酵工艺条件。The feed protein was produced by two different fermentations of Thermoactinomyces vulgaris,Geotrichum candidum and Lactobacillus plantarum in order to make rational use of the production waste of craft beer's grains and blueberry residue,the effects of fermentation time,strain ratio,the ratio and mixing amount of craft beer's grains to blueberry residue,the amount of gluten and inoculum on the the content of the product's true protein were studied.By Plackett-Burman and Box-Behnken design and response surface analysis,the results showed that the optimum fermentation conditions were as follows:the ratio of craft beer's grains to blueberry residue was 1.42∶1(mass ratio),the mixing amount of craft beer's grains and blueberry residue was 87.66%,and the amount of bran was 9.9%.The mass ratio of Trichoderma albicans to Lactobacillus plantarum was 4∶1,and the true protein content could reach 40.15%when the optimum inoculation amount of bacteria was 5%.The quadratic polynomial model between the true protein content of the product and each factor was obtained.The model was verified by the experimental results.The actual value was very close to the theoretical value.The model had high reliability and could predict the fermentation conditions.

关 键 词：精酿啤酒糟 蓝莓果渣 PLACKETT-BURMAN设计 响应面分析 混合发酵 饲料蛋白 

分 类 号：TS209[轻工技术与工程—食品科学] TS261.9[轻工技术与工程—食品科学与工程] S816.6[农业科学—饲料科学]