高尿酸血症对小鼠短期牙周感染牙槽骨破坏的micro⁃CT分析  

Micro⁃CT analysis of hyperuricemia on alveolar bone destruction caused by short⁃term periodontal infection in mice

作　　者：罗咏熙 黄雪莹[1] 冼若婷 余皖鑫 梁莉欣 梁兆佳 陈紫韵 侯丹 余挺[1] LUO Yongxi;HUANG Xueying;XIAN Ruoting;YU Wanxin;LIANG Lixin;LIANG Zhaojia;CHEN Ziyun;HOU Dan;YU Ting(Stomatology Hospital of Guangzhou Medical University,Key Laboratory of Oral Medicine,Guangzhou Institute of Oral Disease,Guangzhou 510182,China)

机构地区：[1]广州医科大学附属口腔医院·口腔医学重点实验室·广州口腔疾病研究所,广东广州510182

出　　处：《口腔疾病防治》2021年第2期88-93,共6页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金项目(81700985);广东省医学科研基金项目(B2020209);大学生创新创业训练计划(201910570014,S201910570040);广州医科大学大学生实验室开放项目(C195015024)。

摘　　要：目的建立高尿酸血症(hyperuricemia,HU)与牙周炎的复合模型,并探索二者是否相关,为牙周炎治疗提供基础。方法将14只雄性KM小鼠分为两组,HU组(n=7)用添加氧嗪酸钾和尿酸的饲料喂养,NC组(n=7)用标准饲料喂养,诱导期为35 d。25 d时,结扎一侧磨牙诱导牙周炎(P侧),对侧不结扎作为对照(C侧)。检测基线及终点血清尿酸(uric acid,UA)浓度,牙槽骨吸收行micro⁃CT分析。结果HU组小鼠的血清UA浓度为(112.94±26.82)μmol/L,NC组血清UA浓度为(72.21±19.95)μmol/L,差异有统计学意义(P<0.05)。HU组与NC组P侧骨体积分数为(29.01±11.09)%、(29.56±15.27)%,差异无统计学意义(t=-0.072,P=0.944);HU组与NC组P侧骨矿物质密度为(0.53±0.16)g/cm3、(0.52±0.14)g/cm3,差异无统计学意义(t=0.038,P=0.970)。血清UA浓度与牙槽骨吸收无相关性(P>0.05)。结论本研究建立HU与牙周炎的联合模型,基于micro⁃CT的颌骨分析尚未发现HU或UA水平与牙周炎相关。Objective Establish a murine model for hyperuricemia(HU) and periodontitis to explore whether there is correlation between them and provide a basis for periodontal treatment.Methods Fourteen male KM mice were divided into 2 groups;the HU group(n=7) was fed food supplemented with potassium oxonate and uric acid,the NC group(n=7) was fed standard food,and the induction period was 35 days.On the 25th day,the molars on one side were ligated to induce periodontitis(P side),while the opposite was true for the control(C side).Baseline and terminal serum uric acid(UA) levels were detected,and alveolar bone resorption was analyzed by micro-CT.Results The serum UA level of HU mice was(112.94±26.82)mol/L,that of the NC group was(72.21±19.95) μmol/L,and the difference in UA level was statistically significant(P <0.05).The P side bone volume fractions of the HU and NC groups were(29.01±11.09)% and(29.56±15.27)%,respectively,which were not significantly different(t=-0.072,P=0.944).The P side bone mineral densities of the HU and NC groups were(0.53±0.16) g/cm3 and(0.52±0.14) g/cm3,respectively,which were not significantly different(t=0.038,P=0.970).Additionally,there was no correlation between HU or serum UA and alveolar bone resorption(P> 0.05).Conclusion This research established a murine model for HU and periodontitis,but based on micro-CT analysis of alveolar bone,no relationship between HU or UA levels and periodontitis was found.

关 键 词：高尿酸血症 尿酸 牙周炎 小鼠模型 牙槽骨吸收 micro⁃CT 骨体积分数 骨矿物质密度 

分 类 号：R781[医药卫生—口腔医学]