一种基于金属薄膜柔性电极的海藻糖定量检测方法研究  

A Method of Trehalose Quantitative Based on Flexible Metal Electrode

作　　者：方旭东 宁景苑 梅正昊 张飞翔 朱博威 阮肖镕 郜园园 易晓梅 惠国华 FANG Xudong;NING Jingyuan;MEI Zhenghao;ZHANG Feixiang;ZHU Bowei;RUAN Xiaorong;GAO Yuanyuan;YI Xiaomei;HUI Guohua(School of Information Engineering,Key Laboratory of Forestry Sensing Technology and Intelligent Equipment of Department of Forestry,Key Laboratory of Forestry Intelligent Monitoring and Information Technology of Zhejiang Province,Zhejiang A&F University,Hangzhou Zhejiang 311300,China)

机构地区：[1]浙江农林大学信息工程学院,林业感知技术与智能装备国家林业局重点实验室,浙江省林业智能监测重点实验室,浙江杭州311300

出　　处：《传感技术学报》2020年第10期1386-1390,共5页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金项目(U1709212);浙江省科技计划项目(2019C02075,LGG18F030012,LGG19F010012);浙江省自然基金项目(LY19F030023)。

摘　　要：本文采用柔性金属薄膜作为工作电极,Ag/AgCl(饱和KCl)电极作为参比电极,铂片电极作为对电极,采用伏安循环法(CV)和电流-时间法(i-t)检测了不同浓度海藻糖的电化学响应,并通过对数据的具体计算分析进行定量检测。结果证明所建立的检测方法灵敏度达0.1917 mA·cm^-2·mmol·L^-1,检测限达2.8244×10^-4 mmol·L^-1(S/N=3)。本传感器具有特异性好、灵敏度高、检测限低、响应速度快、检测方便等优点。柔性金属材料的使用为结构紧凑、可穿戴式微型电化学检测装备研发提供了一种新的思路。A three-electrode electrochemical detection system was constructed with flexible metal film was the working electrode,Ag/AgCl(saturated KCl)electrode as the reference electrode and Pt electrode as the counter electrode.The trehalose samples with different concentrations were measured by cyclic voltammetry(CV)and amperometric i-t curve(i-t).And through the calculation and analysis of the data to carry out quantitative detection.The sensitivity of the method was 0.1917 mA·cm^-2·mmol·L^-1,and the detection limit was 2.8244×10^-4 mmol·L-1(S/N=3).The sensor had the advantages of good specificity,high sensitivity,low detection limit,quick response and convenient detection.Using flexible metal materials provides a new idea for the research and development of compact wearable micro-electrochemical detection equipment.

关 键 词：电化学 定量检测 柔性材料 金属薄膜 海藻糖 

分 类 号：TP212.6[自动化与计算机技术—检测技术与自动化装置]